Biochemistry (Moscow)

, Volume 73, Issue 9, pp 1018–1024 | Cite as

Sulfated galactofucan from Lobophora variegata: Anticoagulant and anti-inflammatory properties

  • V. P. Medeiros
  • K. C. S. Queiroz
  • M. L. Cardoso
  • G. R. G. Monteiro
  • F. W. Oliveira
  • S. F. Chavante
  • L. A. Guimaraes
  • H. A. O. Rocha
  • E. L. LeiteEmail author


Sulfated polysaccharides (fucans and fucoidans) from brown algae show several biological activities, including anticoagulant and anti-inflammatory activities. We have extracted a sulfated heterofucan from the brown seaweed Lobophora variegata by proteolytic digestion, followed by acetone fractionation, molecular sieving, and ion-exchange chromatography. Chemical analyses and 13C-NMR and IR spectroscopy showed that this fucoidan is composed of fucose, galactose, and sulfate at molar ratios of 1:3:2. We compared the anticoagulant activity of L. variegata fucoidan with those of a commercial sulfated polysaccharide (also named fucoidan) from Fucus vesiculosus and heparin. The experimental inflammation models utilized in this work revealed that fucoidan from L. variegata inhibits leukocyte migration to the inflammation site. Ear swelling caused by croton oil was also inhibited when sulfated polysaccharides from F. vesiculosus and L. variegata were used. The precise mechanism of different action between homo-and heterofucans is not clear; nevertheless, the polysaccharides studied here may have therapeutic potential in inflammatory disorders.

Key words

brown algae Lobophora variegata fucan fucoidan anti-clotting activity anti-inflammatory activity 



activated partial thromboplastin time


irritant contact dermatitis


prothrombin time


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  1. 1.
    Berteau, O., and Mulloy, B. (2003) Glycobiology, 13, 29–40.CrossRefGoogle Scholar
  2. 2.
    Mourao, P. A. S. (2007) Braz. J. Med. Biol. Res., 40, 5–17.PubMedCrossRefGoogle Scholar
  3. 3.
    Percival, E., and McDowell, R. H. (1967) Chemistry and Enzymology of Marine Algal Polysaccharides, Academic Press, London, pp. 219–226.Google Scholar
  4. 4.
    Patankar, M. S., Oehninger, L., Barnett, T., Williams, R. L., and Clark, G. F. (1993) J. Biol. Chem., 268, 21770–21776.PubMedGoogle Scholar
  5. 5.
    Bilan, M. I., Grachev, A. A., Shashkov, A. S., Nifantiev, N. E., and Usov, A. I. (2006) Carbohydr. Res., 341, 238–245.PubMedCrossRefGoogle Scholar
  6. 6.
    Bilan, M. I., Grachev, A. A., Ustuzhanina, N. E., Shasshkov, A. S., Nifantiev, E. N., and Usov, A. I. (2001) Carbohydr. Res., 337, 719–730.CrossRefGoogle Scholar
  7. 7.
    Leite, E. L., Medeiros, M. G. L., Rocha, H. A. O., Farias, G. G. M., Silva, L. F., Chavante, S. F., Dietrich, C. P., and Nader, H. B. (1998) Plant Sci., 132, 215–228.CrossRefGoogle Scholar
  8. 8.
    Nader, H. B., Lopes, C. C., Rocha, H. A. O., Santos, E. A., and Dietrich, C. P. (2004) Curr. Pharm. Des., 10, 951–966.PubMedCrossRefGoogle Scholar
  9. 9.
    Yarema, K. J., and Bertozzi, C. R. (1998) Curr. Opin. Chem. Biol., 121, 49–61.CrossRefGoogle Scholar
  10. 10.
    Blondin, C., Fischer, A. M., Boisson-Vidal, C. M., Kazatchkine, D., and Jozefonvicz, J. (1994) Molec. Immun., 31, 247–253.PubMedCrossRefGoogle Scholar
  11. 11.
    Mauray, S., Sternberg, C., Theveniaux, J., Millet, J. C., Sinquin, J., Tapon, B., and Fischer, A. M. (1995) Thromb. Haemost., 74, 1280–1285.PubMedGoogle Scholar
  12. 12.
    Rocha, H. A. O., Moraes, F. A., Trindade, E. S., Franco, C. R. C., Torquato, J. S., Veiga, S. S., Valente, A. P., Mourao, P. A. S., Leite, E. L., Nader, H. B., and Dietrich, C. P. (2005) J. Biol. Chem., 280, 41278–41288.PubMedCrossRefGoogle Scholar
  13. 13.
    Ponce, N. M. A., Pujol, C. A., Damonte, E. B., Flores, M. L., and Stortz, C. A. (2003) Carbohydr. Res., 338, 153–165.PubMedCrossRefGoogle Scholar
  14. 14.
    Patel, M. K., Mulloy, B., Gallagher, K. L., O’Brien, L., and Hughes, A. D. (2002) Thromb. Haemost., 87, 149–154.PubMedGoogle Scholar
  15. 15.
    Pereira, M. S., Mulloy, B., and Mourao, P. A. S. (1999) J. Biol. Chem., 274, 7656–7667.PubMedCrossRefGoogle Scholar
  16. 16.
    Chevolot, L., Foucault, A., Chaubet, F., Kervarec, N., Sinquin, C., Fisher, A. C., and Boisson-Vidal, C. M. (1999) Carbohydr. Res., 319, 154–165.PubMedCrossRefGoogle Scholar
  17. 17.
    Albuquerque, I. R. L., Queiroz, K. C. S., Guimaraes, L. A., Santos, E. A., Leite, E. L., and Rocha, H. A. O. (2004) Braz. J. Med. Biol. Res., 37, 167–171.PubMedCrossRefGoogle Scholar
  18. 18.
    Beijering, R. J. R., Cate, H., and Cate, J. W. (1996) Ann. Hematol., 72, 177–183.PubMedCrossRefGoogle Scholar
  19. 19.
    Rosen, S. D. (2004) Ann. Rev. Immunol., 22, 129–156.CrossRefGoogle Scholar
  20. 20.
    Kansas, G. S. (1996) Blood, 88, 3259–3287.PubMedGoogle Scholar
  21. 21.
    Khan, A. I., Landis, R. C., and Malhotra, R. (2003) Inflammation, 27, 265–280.PubMedCrossRefGoogle Scholar
  22. 22.
    Lehmann, J. C., Jablonski-Westrich, D., Haubold, U., Gutierrez-Ramos, J. C., Springerand, T., and Hamann, A. (2003) J. Immunol., 171, 2588–2593.PubMedGoogle Scholar
  23. 23.
    Silva, T. M. A., Alves, L. G., Queiroz, K. C. S., Santos, M. G. L., Marques, C. T., Chavante, S. F., Rocha, H. A. O., and Leite, E. L. (2005) Braz. J. Med. Biol. Res., 38, 523–533.PubMedGoogle Scholar
  24. 24.
    Dische, Z. (1962) in Methods of Carbohydrate Chemistry (Whistler, R. L., and Wolfrom, M. L., eds.) Vol. 1, Academic Press, London, pp. 478–481.Google Scholar
  25. 25.
    Dische, Z. (1962) in Methods of Carbohydrate Chemistry (Whistler, R. L., and Wolfrom, M. L., eds.) Vol. 1, Academic Press, London, pp. 497–501.Google Scholar
  26. 26.
    Dische, Z. (1962) in Methods of Carbohydrate Chemistry (Whistler, R. L., and Wolfrom, M. L., eds.) Vol. 1, Academic Press, London, pp. 484–488.Google Scholar
  27. 27.
    Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., and Smith, F. (1956) Analyt. Chem., 28, 350–356.CrossRefGoogle Scholar
  28. 28.
    Dodgson, K. S., and Price, R. G. (1962) Biochem. J., 84, 106–110.PubMedGoogle Scholar
  29. 29.
    Lowry, O. H., Farr, A. L., Rosebrough, N. J., and Randall, R. J. (1951) J. Biol. Chem., 193, 265–275.PubMedGoogle Scholar
  30. 30.
    Wang, L., Brown, J. R., Varki, A., and Esko, J. D. (2002) J. Clin. Invest., 10, 127–136.Google Scholar
  31. 31.
    Kloareg, B., and Quatrano, R. S. (1998) Oceanogr. Marine Biol. Annu. Rev., 26, 259–315.Google Scholar
  32. 32.
    Cumashi, A., Ushakova, N. A., Preobrazhenskaya, M. E., Incecco, A., Piccoli, A., Totani, L., Tinari, N., Morozevich, G. E., Berman, A. E., Bilan, M. I., Usov, A. I., Ustyuzhanina, N. E., Grachev, A. A., Sanderson, C. J., Maeve, K., Rabinovich, G. A., Iacobelli, S., and Nifantiev, N. E. (2007) Glycobiology, 17, 541–552.PubMedCrossRefGoogle Scholar
  33. 33.
    Nasu, T., Fukuda, Y., Nagahira, K., Kawashima, H., Noguchi, C., and Nakanishi, T. (1997) Immunol. Lett., 59, 47–51.PubMedCrossRefGoogle Scholar
  34. 34.
    Preobrazhenskaya, M. E., Berman, A. E., Mikhailov, V. I., Ushakova, N. A., Mazurov, A. V., Semenov, A. V., Usov, A. I., Nifantev, N. E., and Bovin, N. V. (1997) Biochem. Mol. Biol. Int., 43, 443–451.PubMedGoogle Scholar

Copyright information

© MAIK Nauka 2008

Authors and Affiliations

  • V. P. Medeiros
    • 1
  • K. C. S. Queiroz
    • 2
  • M. L. Cardoso
    • 1
  • G. R. G. Monteiro
    • 1
  • F. W. Oliveira
    • 1
  • S. F. Chavante
    • 1
  • L. A. Guimaraes
    • 1
  • H. A. O. Rocha
    • 1
  • E. L. Leite
    • 1
    Email author
  1. 1.Departamento de Bioquimica, Centro de BiocienciasUniversidade Federal do Rio Grande do NorteNatalBrazil
  2. 2.Departamento de Biofisica e FarmacologiaUniversidade Federal do Rio Grande do NorteNatalBrazil

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