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Biochemistry (Moscow)

, Volume 73, Issue 4, pp 463–469 | Cite as

Participation of oxygen in the bacterial transformation of 2,4,6-trinitrotoluene

  • E. A. Naumenko
  • A. V. Naumov
  • E. S. Suvorova
  • R. Gerlach
  • A. M. Ziganshin
  • A. P. Lozhkin
  • N. I. Silkin
  • R. P. NaumovaEmail author
Article

Abstract

The exposure of Bacillus cereus ZS18 cell suspensions to 2,4,6-trinitrotoluene (TNT) in the absence of other oxidizable substrates increases oxygen uptake, exceeding the basal level of respiration of the bacterium 1.5- and 2-fold with 50 and 100 mg/liter of TNT, respectively. The interaction of both living and to less extent dead bacterial cells with TNT results in the accumulation of superoxide anion (\( O_2^{\dot ---} \) ) in the extracellular medium, which was revealed by the EPR spectroscopy. The accumulation of \( O_2^{\dot ---} \) decreased by 50–70% in the presence of Cu,Zn-superoxide dismutase of animal origin. In the presence of living bacterial cells, the level of TNT decreased progressively, yielding hydroxylaminodinitrotoluenes together with \( O_2^{\dot ---} \) . In the presence of heat-killed cells, a moderate decrease in TNT was observed, and the appearance of \( O_2^{\dot ---} \) was not accompanied by the production of any detectable TNT metabolites. Chelating agents inhibited the transformation of TNT and decreased the formation of \( O_2^{\dot ---} \) . The demonstrated generation of \( O_2^{\dot ---} \) during the interaction of TNT with K4[Fe(CN)6] together with the observed effects of chelating agents suggest the participation of iron in the one-electron reduction of TNT and the functioning of an extracellular redox cycle with the involvement of molecular oxygen.

Key words

2,4,6-trinitrotoluene aerobic transformation superoxide anion 

Abbreviations

ADNT

aminodinitrotoluene

HADNT

hydroxylaminodinitrotoluene

\( O_2^{\dot ---} \)

superoxide anion

SOD

Cu,Zn-superoxide dismutase

TNT

2,4,6-trinitrotoluene

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Copyright information

© MAIK Nauka 2008

Authors and Affiliations

  • E. A. Naumenko
    • 1
  • A. V. Naumov
    • 2
  • E. S. Suvorova
    • 2
  • R. Gerlach
    • 2
  • A. M. Ziganshin
    • 1
  • A. P. Lozhkin
    • 1
  • N. I. Silkin
    • 1
  • R. P. Naumova
    • 1
    Email author
  1. 1.Kazan State UniversityKazanRussia
  2. 2.Montana State UniversityBozemanUSA

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