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Biochemistry (Moscow)

, Volume 73, Issue 4, pp 427–431 | Cite as

Influence of gel dimensions on resolution and sample throughput on two-dimensional gels

  • KiBeom LeeEmail author
  • KyungBae Pi
  • Hong-Gu LeeEmail author
Article

Abstract

To achieve high throughput and economical format of 2-D PAGE, comparison between gel size and resolution was conducted on human breast carcinoma cell line (MCF-7/AZ) proteins. SDS gel length showed a weaker influence of separation length on resolution in the second dimension, and there was little benefit of separation distances greater than 15 to 19 cm. IPG strip separation distances were very important with dramatic increase in resolution of longer gels compared with smaller gels, and maximal resolution was obtained using 18-and 24-cm IPG strips. Loading optimal amount of proteins on 2-D gels can also increase the number of detected spots. Therefore, taken together, compromise 2-D gels are crucial for higher capacity and higher throughput.

Key words

two-dimensional polyacrylamide gel electrophoresis immobilized pH gradient (IPG) strip length SDS gel length/high throughput resolution 

Abbreviations

2-D PAGE

two-dimensional polyacrylamide gel electrophoresis

IEF

isoelectrofocusing

IPG

immobilized pH gradient

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Copyright information

© MAIK Nauka 2008

Authors and Affiliations

  1. 1.Department of BiotechnologySongDo TechnoParkIncheonKorea
  2. 2.Research Institute, AdipoGenKorea University, College of Life Science and BiotechnologySeoulKorea
  3. 3.School of Agricultural BiotechnologySeoul National UniversitySeoulKorea

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