Biochemistry (Moscow)

, Volume 73, Issue 3, pp 353–357 | Cite as

Human heart LIM protein has transcription activation ability related to LIM domain 1

  • Bin Zheng
  • Mei Han
  • Jin-Kun WenEmail author


Human heart LIM (hhLIM), a muscle-specific expressed transcriptional coactivator of cardiac hypertrophy related gene, stimulates transcriptional activation of the ANF gene promoter in H9C2 cells. We revealed that the N-terminal half of hhLIM mediated this activation, in which the LIM domain 1 and protein kinase C phosphorylation site are important, especially the LIM domain 1. Further, mutagenesis of the conserved Cys in the LIM domain 1 abolished its ability to activate cardiac hypertrophy. These findings suggest that hhLIM is a typical LIM family member with powerful transcription activation.

Key words

hhLIM LIM domain cardiac hypertrophy ANF 



atrial natriuretic factor


human heart LIM protein


polymerase chain reaction


protein kinase C


thymidine kinase


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Copyright information

© Pleiades Publishing, Ltd. 2008

Authors and Affiliations

  1. 1.Department of BiochemistryHebei Medical UniversityShijiazhuangChina

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