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Biochemistry (Moscow)

, Volume 73, Issue 3, pp 353–357 | Cite as

Human heart LIM protein has transcription activation ability related to LIM domain 1

  • Bin Zheng
  • Mei Han
  • Jin-Kun WenEmail author
Article

Abstract

Human heart LIM (hhLIM), a muscle-specific expressed transcriptional coactivator of cardiac hypertrophy related gene, stimulates transcriptional activation of the ANF gene promoter in H9C2 cells. We revealed that the N-terminal half of hhLIM mediated this activation, in which the LIM domain 1 and protein kinase C phosphorylation site are important, especially the LIM domain 1. Further, mutagenesis of the conserved Cys in the LIM domain 1 abolished its ability to activate cardiac hypertrophy. These findings suggest that hhLIM is a typical LIM family member with powerful transcription activation.

Key words

hhLIM LIM domain cardiac hypertrophy ANF 

Abbreviations

ANF

atrial natriuretic factor

hhLIM

human heart LIM protein

PCR

polymerase chain reaction

PKC

protein kinase C

TK

thymidine kinase

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Copyright information

© Pleiades Publishing, Ltd. 2008

Authors and Affiliations

  1. 1.Department of BiochemistryHebei Medical UniversityShijiazhuangChina

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