Human heart LIM protein has transcription activation ability related to LIM domain 1
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Human heart LIM (hhLIM), a muscle-specific expressed transcriptional coactivator of cardiac hypertrophy related gene, stimulates transcriptional activation of the ANF gene promoter in H9C2 cells. We revealed that the N-terminal half of hhLIM mediated this activation, in which the LIM domain 1 and protein kinase C phosphorylation site are important, especially the LIM domain 1. Further, mutagenesis of the conserved Cys in the LIM domain 1 abolished its ability to activate cardiac hypertrophy. These findings suggest that hhLIM is a typical LIM family member with powerful transcription activation.
Key wordshhLIM LIM domain cardiac hypertrophy ANF
atrial natriuretic factor
human heart LIM protein
polymerase chain reaction
protein kinase C
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- 3.Chen, H., Zhou, Z., Zhang, J. F., and Zhou, A. R. (2000) Chin. J. Biochem. Mol. Biol., 16, 295–300.Google Scholar