Biochemistry (Moscow)

, Volume 73, Issue 3, pp 346–352 | Cite as

Evolution of DNA polymerase ι structure and function in eukaryotes

  • A. V. MakarovaEmail author
  • V. Z. Tarantul
  • L. V. Gening


Analysis of DNA polymerase ι (Pol ι) enzymic activity in different classes of eukaryotes has shown that error-prone activity of this enzyme can be found only in mammals, and that it is completely absent from organisms that are at lower stages of development. It was supposed that the emergence of the error-prone Pol ι activity in mammals is caused by structural alteration of the active center. Possible functions of error-prone Pol ι in higher eukaryotes are discussed.

Key words

DNA polymerase ι error-prone activity hypermutagenesis 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Haracska, L., Prakash, L., and Prakash, S. (2002) J. Biol. Chem., 277, 15546–15551.PubMedCrossRefGoogle Scholar
  2. 2.
    Johnson, R. E., Washington, M. T., Haracska, L., Prakash, S., and Prakash, L. (2000) Nature, 406, 1015–1019.PubMedCrossRefGoogle Scholar
  3. 3.
    Matsuda, T., Bebenek, K., Masutani, C., Hanaoka, F., and Kunkel, T. A. (2000) Nature, 404, 1011–1013.PubMedCrossRefGoogle Scholar
  4. 4.
    Ohashi, E., Bebenek, K., Matsuda, T., Feaver, W. J., Gerlach, V. L., Friedberg, E. C., Ohmori, H., and Kunkel, T. A. (2000) Biol. Chem., 275, 39678–39684.CrossRefGoogle Scholar
  5. 5.
    Tissier, A., McDonald, J. P., Frank, E. G., and Woodgate, R. (2000) Genes Dev., 14, 1642–1650.PubMedGoogle Scholar
  6. 6.
    Zhang, Y., Yuan, F., Wu, X., and Wang, Z. (2000) Mol. Cell. Biol., 20, 7099–7108.PubMedCrossRefGoogle Scholar
  7. 7.
    Vaisman, A., Frank, E. G., McDonald, J. P., Tissier, A., and Woodgate, R. (2002) Mutat. Res., 510, 9–22.PubMedCrossRefGoogle Scholar
  8. 8.
    Bebenek, K., Tissier, A. K., Frank, E. G., McDonald, J. P., Prasad, S., Wilson, S. H., Woodgate, R., and Kunkel, T. A. (2001) Science, 291, 2156–2159.PubMedCrossRefGoogle Scholar
  9. 9.
    Vaisman, A., Takasawa, K., Iwai, S., and Woodgate, R. (2006) DNA Repair (Amst.), 5, 210–218.CrossRefGoogle Scholar
  10. 10.
    Vaisman, A., and Woodgate, R. (2001) EMBO J., 20, 6520–6529.PubMedCrossRefGoogle Scholar
  11. 11.
    McDonald, J. P., Tissier, A., Frank, E. G., Iwai, S., Hanaoka, F., and Woodgate, R. (2001) Philos. Trans. Roy. Soc. (Lond. B Biol. Sci.), 356, 53–60.CrossRefGoogle Scholar
  12. 12.
    Washington, M. T., Johnson, R. E., Prakash, L., and Prakash, S. (2004) Mol. Cell. Biol., 24, 936–943.PubMedCrossRefGoogle Scholar
  13. 13.
    Ishikava, T., Uematsu, N., Mizukoshi, T., Iwai, S., Masutani, C., Hanaoka, F., Ueda, R., Ohmori, H., and Todo, H. (2001) J. Biol. Chem., 276, 15155–15163.CrossRefGoogle Scholar
  14. 14.
    McDonald, J. P., Frank, E. G., Plosky, B. S., Rogozin, I. B., Masutani, C., Hanaoka, F., Woodgate, R., and Gearhart, P.J. (2003) J. Exp. Med., 198, 635–643.PubMedCrossRefGoogle Scholar
  15. 15.
    Wang, M., Devereux, T. R., Vikis, H. G., McCulloch, S. D., Holliday, W., Anna, C., Wang, Y., Bebenek, K., Kunkel, T.A., Guan, K., and You, M. (2004) Cancer Res., 64, 1924–1931.PubMedCrossRefGoogle Scholar
  16. 16.
    Gening, L. V., Petrochenkov, A. N., Reshetnyak, A. B., Andreeva, L. E., and Tarantul, V. Z. (2004) Biochemistry (Moscow), 69, 435–440.CrossRefGoogle Scholar
  17. 17.
    Gening, L. V., Makarova, A. V., Malashenko, A. M., and Tarantul, V. Z. (2006) Biochemistry (Moscow), 71, 155–159.CrossRefGoogle Scholar
  18. 18.
    Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W., and Lipman, D. J. (1997) Nucleic Acids Res., 25, 3389–3402.PubMedCrossRefGoogle Scholar
  19. 19.
    Nair, D. T., Johnson, R. E., Prakash, L., and Aggarwal, A. K. (2004) Nature, 430, 377–380.PubMedCrossRefGoogle Scholar
  20. 20.
    Johnson, R. E., Prakash, S., and Prakash, L. (2005) Proc. Natl. Acad. Sci. USA, 102, 10466–10471.PubMedCrossRefGoogle Scholar
  21. 21.
    Galaktionov, V. G. (2005) Evolutionary Immunology [in Russian], Akademkniga, Moscow.Google Scholar
  22. 22.
    Du Pasquier, L. (1982) Nature, 296, 311–313.PubMedCrossRefGoogle Scholar
  23. 23.
    Du Pasquier, L., and Flajnik, M. (1997) Fundamental Immunology (Paul, W., ed.), LW&W.Google Scholar
  24. 24.
    Hsu, E. (1998) Immunol. Rev., 162, 25–36.PubMedCrossRefGoogle Scholar
  25. 25.
    Denepoux, S., Razanajaona, D., Blanchard, D., Meffre, G., and Capra, J. D. (1997) Immunity, 6, 35–46.PubMedCrossRefGoogle Scholar
  26. 26.
    Poltoratsky, V., Woo, C. J., Tippin, B., Martin, A., Goodman, M. F., and Scharff, M. F. (2001) Proc. Natl. Acad. Sci. USA, 98, 7976–7981.PubMedCrossRefGoogle Scholar
  27. 27.
    Faili, A., Aoufouchi, S., Flatter, E., Gueranger, Q., Reunaud, C. A., and Weill, J. C. (2002) Nature, 419, 944–947.PubMedCrossRefGoogle Scholar
  28. 28.
    Marmoto, S. A., Yang, W. W., Vaisman, A., Maas, A., Yokoi, M., Hoeijmakers, J. H., Hanaoka, F., Woodgate, R., and Gearhart, P. J. (2006) DNA Repair (Amst.), 5, 392–398.CrossRefGoogle Scholar
  29. 29.
    Shimizu, T., Azuma, T., Ishiguro, M., Kanjo, N., Yamada, S., and Ohmori, H. (2005) Immunol. Lett., 98, 259–264.PubMedCrossRefGoogle Scholar
  30. 30.
    Gening, L. V., and Tarantul, V. Z. (2006) Immunol. Lett., 106, 198–199.PubMedCrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2008

Authors and Affiliations

  • A. V. Makarova
    • 1
    Email author
  • V. Z. Tarantul
    • 1
  • L. V. Gening
    • 1
  1. 1.Institute of Molecular GeneticsRussian Academy of SciencesMoscowRussia

Personalised recommendations