Biochemistry (Moscow)

, 73:289 | Cite as

Isolation and physicochemical properties of tankyrase of human embryonic kidney cells of line 293

  • N. N. Sidorova
  • A. O. Fadeev
  • A. N. KuimovEmail author


We have isolated and purified endogenous cytosolic tankyrase from human embryonic kidney cells of line 293. Our data confirm a model of De Rycker and Price who consider that tankyrase is a master scaffolding protein capable of regulating assembly of large protein complexes. We have also studied kinetic characteristics of tankyrase in the complex, pH dependence of the enzyme activity, and its physicochemical properties.

Key words

tankyrase poly(ADP-ribose) PARP ADP-ribosylation kidney 



poly(ADP-ribose) polymerase


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Copyright information

© Pleiades Publishing, Ltd. 2008

Authors and Affiliations

  1. 1.Belozersky Institute of Physico-Chemical BiologyLomonosov Moscow State UniversityMoscowRussia
  2. 2.Faculty of Bioengineering and BioinformaticsLomonosov Moscow State UniversityMoscowRussia

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