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Biochemistry (Moscow)

, Volume 73, Issue 3, pp 283–288 | Cite as

Lipid rafts association of synaptotagmin I on synaptic vesicles

  • Ji-Hua Lv
  • Li He
  • Sen-Fang SuiEmail author
Article

Abstract

We confirmed the raft association of synaptotagmin I (syt I) in synaptic vesicles by sucrose density gradient centrifugation, cholesterol depletion, and temperature dependence, and Ca2+ was found to positively regulate this association. Furthermore, using syt I mutants we found that the transmembrane domain (TMD) of syt I plays an important role in localizing syt I into the lipid rafts of synaptic vesicles, and the raft association of the TMD can be regulated by its phosphorylation status.

Key words

lipid rafts synaptic vesicles synaptotagmin I 

Abbreviations

MbCD

methyl-β-cyclodextrin

SNAP-25

synaptosomal-associated protein of 25 kD

SNARES

soluble N-ethylmaleimide-sensitive factor attachment protein receptors

syt I

synaptotagmin I

TMD

transmembrane domain of syt I

VAMP

vesicle-associated membrane protein

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Copyright information

© Pleiades Publishing, Ltd. 2008

Authors and Affiliations

  1. 1.Department of Biological Sciences and Biotechnology, State-Key Laboratory of Biomembrane and Membrane BiotechnologyTsinghua UniversityBeijingChina

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