Advertisement

Biochemistry (Moscow)

, Volume 73, Issue 3, pp 278–282 | Cite as

Membrane localization of the MAK-V protein kinase

  • S. V. Kalinichenko
  • E. V. Korobko
  • I. V. KorobkoEmail author
Article

Abstract

Activities of many proteins including protein kinases are often regulated by their dynamic association with specific intracellular compartments. MAK-V is an AMPK-like protein kinase with poorly characterized functions and mechanisms of action. Similarly to many other protein kinases, association of MAK-V with specific intracellular compartments could be essential for its proper functions. In this work, we studied subcellular distribution of exogenously produced and endogenous MAK-V proteins in mammalian cells using biochemical cell fractioning aiming to supplement data on MAK-V intracellular localization studied by immunocytochemical methods. We found that a significant portion of MAK-V protein in mammalian cells is associated with membranes. Moreover, MAK-V expressed in yeast was also targeted to membrane, thus suggesting an evolutionarily conservative mechanism of MAK-V membrane association. Based on the ability of various MAK-V deletion mutants to localize to membrane and comparison of MAK-V amino acid sequences from different species, we suggest a possible mechanism governing MAK-V association with intracellular membranes.

Key words

protein kinase MAK-V membrane localization ubiquitin-associated domain 

Abbreviations

PI(4,5)P2

phosphatidylinositol-(4,5)-bisphosphate

PNS

postnuclear supernatant

UBA

ubiquitin-associated domain

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Manning, G., Whyte, D. B., Martinez, R., Hunter, T., and Sudarsanam, S. (2002) Science, 298, 1912–1934.PubMedCrossRefGoogle Scholar
  2. 2.
    Ruzov, A. S., Mertsalov, I. B., Meehan, R., Kiselev, S., Buchman, V. L., and Korobko, I. V. (2004) Dev. Genes Evol., 214, 139–143.PubMedCrossRefGoogle Scholar
  3. 3.
    Bradham, C. A., Foltz, K. R., Beane, W. S., Arnone, M. I., Rizzo, F., Coffman, J. A., Mushegian, A., Goel, M., Morales, J., Geneviere, A. M., Lapraz, F., Robertson, A. J., Kelkar, H., Loza-Coll, M., Townley, I. K., Raisch, M., Roux, M. M., Lepage, T., Gache, C., McClay, D. R., and Manning, G. (2006) Dev. Biol., 300, 180–193.PubMedCrossRefGoogle Scholar
  4. 4.
    Korobko, I. V., Kabishev, A. A., and Kiselev, S. L. (1997) Dokl. Akad. Nauk, 354, 554–556.PubMedGoogle Scholar
  5. 5.
    Korobko, I. V., Korobko, E. V., and Kiselev, S. L. (2000) Mol. Gen. Genet., 264, 411–418.PubMedCrossRefGoogle Scholar
  6. 6.
    Gardner, H. P., Wertheim, G. B., Ha, S. I., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., Marquis, S. T., and Chodosh, L. A. (2000) Genomics, 63, 46–59.PubMedCrossRefGoogle Scholar
  7. 7.
    Reymond, A., Marigo, V., Yaylaoglu, M. B., Leoni, A., Ucla, C., Scamuffa, N., Caccioppoli, C., Dermitzakis, E. T., Lyle, R., Banfi, S., Eichele, G., Antonarakis, S. E., and Ballabio, A. (2002) Nature, 420, 582–586.PubMedCrossRefGoogle Scholar
  8. 8.
    Korobko, E. V., Kiselev, S. L., and Korobko, I. V. (2004) Cell Biol. Int., 28, 49–56.PubMedCrossRefGoogle Scholar
  9. 9.
    Sakai, M., Tamura, K., Tsurumi, Y., Tanaka, Y., Koide, Y., Matsuda, M., Ishigami, T., Yabana, M., Tokita, Y., Hiroi, Y., Komuro, I., and Umemura, S. (2007) Am. J. Physiol. Renal. Physiol., 292, F1526–F1536.PubMedCrossRefGoogle Scholar
  10. 10.
    Kiselev, S. L., Kustikova, O. S., Korobko, E. V., Prokhortchouk, E. B., Kabishev, A. A., Lukanidin, E. M., and Georgiev, G. P. (1998) J. Biol. Chem., 273, 18633–18639.PubMedCrossRefGoogle Scholar
  11. 11.
    Korobko, I. V., Korobko, E. V., Ninkina, N. N., Buchman, V. L., and Kiselev, S. L. (2007) Dokl. Akad. Nauk, 412, 37–39.Google Scholar
  12. 12.
    Stenmark, H., Vitale, G., Ullrich, O., and Zerial, M. (1995) Cell, 83, 423–432.PubMedCrossRefGoogle Scholar
  13. 13.
    Korobko, I. V., Zavalishina, L. E., Kiselev, S. L., Raikhlin, N. T., and Frank, G. A. (2004) Arkh. Patol., 66, 6–9.PubMedGoogle Scholar
  14. 14.
    Drenan, R. M., Liu, X., Bertram, P. G., and Zheng, X. F. (2004) J. Biol. Chem., 279, 772–778.PubMedCrossRefGoogle Scholar
  15. 15.
    Plant, P. J., Yeger, H., Staub, O., Howard, P., and Rotin, D. (1997) J. Biol. Chem., 272, 32329–32336.PubMedCrossRefGoogle Scholar
  16. 16.
    Suzuki, A., Hirata, M., Kamimura, K., Maniwa, R., Yamanaka, T., Mizuno, K., Kishikawa, M., Hirose, H., Amano, Y., Izumi, N., Miwa, Y., and Ohno, S. (2004) Curr. Biol., 14, 1425–1435.PubMedCrossRefGoogle Scholar
  17. 17.
    Hurov, J. B., Watkins, J. L., and Piwnica-Worms, H. (2004) Curr. Biol., 14, 736–741.PubMedCrossRefGoogle Scholar
  18. 18.
    Rameh, L. E., and Cantley, L. C. (1999) J. Biol. Chem., 274, 8347–8350.PubMedCrossRefGoogle Scholar
  19. 19.
    Toker, A., and Cantley, L. C. (1997) Nature, 387, 673–676.PubMedCrossRefGoogle Scholar
  20. 20.
    Spudich, G., Chibalina, M. V., Au, J. S., Arden, S. D., Buss, F., and Kendrick-Jones, J. (2007) Nat. Cell Biol., 9, 176–183.PubMedCrossRefGoogle Scholar
  21. 21.
    Herrig, A., Janke, M., Austermann, J., Gerke, V., Janshoff, A., and Steinem, C. (2006) Biochemistry, 45, 13025–13034.PubMedCrossRefGoogle Scholar
  22. 22.
    Sanchez-Bautista, S., Marin-Vicente, C., Gomez-Fernandez, J. C., and Corbalan-Garcia, S. (2006) J. Mol. Biol., 362, 901–914.PubMedCrossRefGoogle Scholar
  23. 23.
    Birkeland, H. C., and Stenmark, H. (2004) Curr. Top. Microbiol. Immunol., 282, 89–115.PubMedGoogle Scholar
  24. 24.
    Ching, T. T., Lin, H. P., Yang, C. C., Oliveira, M., Lu, P. J., and Chen, C. S. (2001) J. Biol. Chem., 276, 43932–43938.PubMedCrossRefGoogle Scholar
  25. 25.
    Yu, F. X., Sun, H. Q., Janmey, P. A., and Yin, H. L. (1992) J. Biol. Chem., 267, 14616–14621.PubMedGoogle Scholar
  26. 26.
    Jaleel, M., Villa, F., Deak, M., Toth, R., Prescott, A. R., van Aalten, D. M., and Alessi, D. R. (2006) Biochem. J., 394, 545–555.PubMedCrossRefGoogle Scholar
  27. 27.
    Beullens, M., Vancauwenbergh, S., Morrice, N., Derua, R., Ceulemans, H., Waelkens, E., and Bollen, M. (2005) J. Biol. Chem., 280, 40003–40011.PubMedCrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2008

Authors and Affiliations

  • S. V. Kalinichenko
    • 1
  • E. V. Korobko
    • 1
    • 2
  • I. V. Korobko
    • 1
    • 2
    Email author
  1. 1.Institute of Gene BiologyRussian Academy of SciencesMoscowRussia
  2. 2.University of Oslo, Centre for Medical Studies in RussiaMoscowRussia

Personalised recommendations