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Biochemistry (Moscow)

, 73:273 | Cite as

Ribonuclease, deoxyribonuclease, and antiviral activity of Escherichia coli-expressed Bougainvillea xbuttiana antiviral protein 1

  • N. L. Choudhary
  • O. P. Yadav
  • M. L. LodhaEmail author
Article

Abstract

A full-length cDNA encoding ribosome-inactivating/antiviral protein from the leaves of Bougainvillea xbuttiana was recently isolated. The coding region of cDNA was cloned and expressed in Escherichia coli, and the protein product was designated as BBAP1 (Bougainvillea xbuttiana antiviral protein 1). BBAP1 showed ribonuclease activity against Torula yeast RNA. It also exhibited depurination activity against supercoiled pBlueScript SK+ plasmid DNA in a concentration dependent manner, and was found to convert nicked circular DNA into linear form only at higher concentration. On bioassay, BBAP1 exhibited antiviral activity against sunnhemp rosette virus infecting Cyamopsis tetragonoloba leaves in which 95% inhibition of local lesion formation was observed.

Key words

Bougainvillea xbuttiana antiviral protein BBAP1 RNase activity DNase activity antiviral activity 

Abbreviations

AVP

antiviral protein

BBAP1

Bougainvillea xbuttiana antiviral protein 1

ORF

open reading frame

PAP

pokeweed antiviral protein

RIP

ribosome-inactivating protein

SRV

sunnhemp rosette virus

TMV

tobacco mosaic virus

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Copyright information

© Pleiades Publishing, Ltd. 2008

Authors and Affiliations

  1. 1.Division of BiochemistryIndian Agricultural Research InstituteNew DelhiIndia
  2. 2.Emeritus Scientist, Division of GeneticsIndian Agricultural Research InstituteNew DelhiIndia

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