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Biochemistry (Moscow)

, Volume 73, Issue 2, pp 164–170 | Cite as

Isolation and oligomeric composition of cytochrome c nitrite reductase from the haloalkaliphilic bacterium Thioalkalivibrio nitratireducens

  • T. V. TikhonovaEmail author
  • E. S. Slutskaya
  • A. A. Filimonenkov
  • K. M. Boyko
  • S. Yu. Kleimenov
  • P. V. Konarev
  • K. M. Polyakov
  • D. I. Svergun
  • A. A. Trofimov
  • V. G. Khomenkov
  • R. A. Zvyagilskaya
  • V. O. Popov
Article

Abstract

A new procedure for isolation of cytochrome c nitrite reductase from the haloalkaliphilic bacterium Thioalkalivibrio nitratireducens increasing significantly the yield of the purified enzyme is presented. The enzyme is isolated from the soluble fraction of the cell extract as a hexamer, as shown by gel filtration chromatography and small angle X-ray scattering analysis. Thermostability of the hexameric form of the nitrite reductase is characterized in terms of thermoinactivation and thermodenaturation.

Key words

cytochrome c nitrite reductase isolation oligomeric composition thermostability thermodenaturation 

Abbreviations

NrfA

bacterial cytochrome c-containing nitrite reductases coded by nrfA gene

SAXS

small angle X-ray scattering

TvNiR

cytochrome c nitrite reductase from Thioalkalivibrio nitratireducens

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Copyright information

© Pleiades Publishing, Ltd. 2008

Authors and Affiliations

  • T. V. Tikhonova
    • 1
    Email author
  • E. S. Slutskaya
    • 1
  • A. A. Filimonenkov
    • 1
  • K. M. Boyko
    • 1
  • S. Yu. Kleimenov
    • 1
  • P. V. Konarev
    • 2
    • 3
  • K. M. Polyakov
    • 4
  • D. I. Svergun
    • 2
    • 3
  • A. A. Trofimov
    • 4
  • V. G. Khomenkov
    • 1
  • R. A. Zvyagilskaya
    • 1
  • V. O. Popov
    • 1
  1. 1.Bach Institute of BiochemistryRussian Academy of SciencesMoscowRussia
  2. 2.Shubnikov Institute of CrystallographyRussian Academy of SciencesMoscowRussia
  3. 3.European Molecular Biology Laboratory (EMBL)HamburgGermany
  4. 4.Engelhardt Institute of Molecular BiologyRussian Academy of SciencesMoscowRussia

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