Biochemistry (Moscow)

, Volume 73, Issue 2, pp 139–148 | Cite as

Influence of cultivation conditions on spatial structure and functional activity of OmpF-like porin from outer membrane of Yersinia pseudotuberculosis

  • O. D. Novikova
  • T. I. VakorinaEmail author
  • V. A. Khomenko
  • G. N. Likhatskaya
  • N. Yu. Kim
  • V. I. Emelyanenko
  • S. M. Kuznetsova
  • T. F. Solov’eva


The influence of cultivation conditions of pseudotuberculosis bacteria on the spatial structure and the functional activity of nonspecific OmpF-like porin was studied by means of optical spectroscopy, scanning microcalorimetry, and bilayer lipid membrane technique. With this goal, porin samples isolated from microbial masses grown at different temperatures, nutrient medium densities, and growth phases were characterized. According to CD data, the porin samples under investigation represent β-sheet proteins. It was found that the protein isolated from the colonial culture of pseudotuberculosis bacteria grown at low temperature has the most compact structure. Using intrinsic protein fluorescence, it was shown that different conditions of pseudotuberculosis bacteria cultivation (temperature, medium, growth phase) led to the changes in spectral properties of porin fluorescence due to the redistribution of the contributions of tyrosine and different classes of tryptophan residues to the total protein emission. Heat inactivation of porin samples was studied using CD spectroscopy, intrinsic protein fluorescence, and scanning microcalorimetry. Spatial features of the porin samples were found to affect their functional activities. Considering all these data, it is possible to correlate the spatial structure and functional activity of porin samples isolated under different cultivation conditions of bacteria and the composition of the outer membrane lipid matrix.

Key words

nonspecific OmpF-like porins spatial structure thermostability functional activity lipid matrix 



bilayer lipid membrane






logarithmic growth phase


nutrient agar


nutrient broth


neutral lipids


bacterial outer membrane






peptidoglycan-protein complexes


early stationary growth phase


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Copyright information

© Pleiades Publishing, Ltd. 2008

Authors and Affiliations

  • O. D. Novikova
    • 1
  • T. I. Vakorina
    • 1
    Email author
  • V. A. Khomenko
    • 1
  • G. N. Likhatskaya
    • 1
  • N. Yu. Kim
    • 1
  • V. I. Emelyanenko
    • 2
  • S. M. Kuznetsova
    • 2
  • T. F. Solov’eva
    • 1
  1. 1.Pacific Institute of Bioorganic Chemistry, Far East BranchRussian Academy of SciencesVladivostokRussia
  2. 2.Institute of Theoretical and Experimental BiophysicsRussian Academy of SciencesPushchino, Moscow RegionRussia

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