Light damaging action of all-trans-retinal and its derivatives on rhodopsin molecules in the photoreceptor membrane
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We have reproduced the model system containing A2-rhodopsin, NR-PE, A2-PE, and ATR-dimer-PE in order to study photosensitized damage of rhodopsin within photoreceptor membranes of rod outer segments. We have demonstrated that irradiation of such a system with visible light (400–700 nm) distorts the most important functional property of native visual pigment—its ability to regenerate after addition of 11-cis-retinal in the dark. We have also shown that all-trans-retinal bound to membrane phospholipids and rhodopsin has less photosensitizing activity that free all-trans-retinal.
Key wordsvisual pigment rhodopsin all-trans-retinal photosensitizers photodamage
ATP-binding cassette transporter
rhodopsin which has each of its three lysine residues modified with two all-trans-retinal molecules
rod outer segments
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