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Biochemistry (Moscow)

, Volume 73, Issue 1, pp 65–69 | Cite as

Polyphosphates and exopolyphosphatases in cytosol and mitochondria of Saccharomyces cerevisiae during growth on glucose or ethanol under phosphate surplus

  • N. A. Andreeva
  • T. V. KulakovskayaEmail author
  • E. V. Kulakovskaya
  • I. S. Kulaev
Article

Abstract

Content and chain lengths of inorganic polyphosphates (polyP) as well as exopolyphosphatase activities were compared in cytosol and mitochondria of the yeast Saccharomyces cerevisiae during growth on glucose or ethanol under phosphate surplus. PolyP metabolism in cytosol and mitochondria was substantially dependent upon the carbon source. Acid-soluble polyP accumulated mainly in cytosol using either glucose or ethanol. The level of the accumulation was lower during growth on ethanol compared to that on glucose. Increase in polyP content in mitochondria was observed during growth on glucose, but not on ethanol. In cytosol the activity of exopolyphosphatase PPN1 was increased and the activity of exopolyphosphatase PPX1 was decreased independently of the carbon source under phosphate surplus conditions. Growth on ethanol caused exopolyphosphatase PPN1 to appear in the soluble mitochondrial fraction, while during growth on glucose only exopolyphosphatase PPX1 was present in this fraction.

Key words

inorganic polyphosphates (polyP) exopolyphosphatase cytosol mitochondria phosphate surplus yeast Saccharomyces cerevisiae 

Abbreviations

polyP

polyphosphates

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Copyright information

© Pleiades Publishing, Ltd. 2008

Authors and Affiliations

  • N. A. Andreeva
    • 1
  • T. V. Kulakovskaya
    • 1
    Email author
  • E. V. Kulakovskaya
    • 1
  • I. S. Kulaev
    • 1
  1. 1.Skryabin Institute of Biochemistry and Physiology of MicroorganismsRussian Academy of SciencesPushchino, Moscow RegionRussia

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