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Biochemistry (Moscow)

, Volume 72, Issue 11, pp 1270–1278 | Cite as

β-synuclein protein from Xenopus laevis: Overexpression in Escherichia coli of the GST-tagged protein and production of polyclonal antibodies

  • Zhu Yuan
  • Xinyu Zhao
  • Fei Yan
  • Jian Zhao
  • Huanyi Liu
  • Shaoquan Xiong
  • Jiong Li
  • Lijuan ChenEmail author
  • Yuquan Wei
Article
  • 48 Downloads

Abstract

This report presents a procedure to obtain and purify recombinant β-synuclein from Xenopus laevis expressed in Escherichia coli as a glutathione-S-transferase fusion protein. After identification by mass spectrometry, the protein was then used to raise anti-X. laevis β-synuclein polyclonal antibodies, which were suitable to detect the presence of β-synuclein in X. laevis brain by Western blot. This is the first report of a positive identification of β-synuclein in an amphibian at the protein level.

Key words

Xenopus laevis β-synuclein Escherichia coli β-synuclein expression antibody production 

Abbreviations

anti-Xen-SYNB polyclonal antibodies

anti-X. laevis β-synuclein polyclonal antibodies

ESI mass spectrometry

electrospray ionization mass spectrometry

GST

glutathione-S-transferase

IPTG

isopropyl-β-D-thiogalactopyranoside

ORF

open reading frame

PVDF

polyvinylidene fluoride

SYNB

β-synuclein

xSYNB

Xenopus laevis β-synuclein

Xen-GST-SYNB

X. laevis GST-β-synuclein fusion protein

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Copyright information

© Pleiades Publishing, Ltd. 2007

Authors and Affiliations

  • Zhu Yuan
    • 1
    • 2
  • Xinyu Zhao
    • 1
  • Fei Yan
    • 1
  • Jian Zhao
    • 1
  • Huanyi Liu
    • 1
  • Shaoquan Xiong
    • 1
  • Jiong Li
    • 1
  • Lijuan Chen
    • 1
    Email author
  • Yuquan Wei
    • 1
  1. 1.State Key Laboratory of Biotherapy, West China Hospital, West China Medical School and College of Life ScienceSichuan UniversityChengduChina
  2. 2.Department of ImmunologyChengdu Medical CollegeChengduChina

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