Biochemistry (Moscow)

, Volume 72, Issue 11, pp 1194–1198 | Cite as

Gene expression for VEGF-A, VEGF-C, and their receptors in murine lymphocytes and macrophages

  • O. I. Stepanova
  • A. V. Krylov
  • V. I. Lioudyno
  • E. P. Kisseleva
Accelerated Publication

Abstract

Vascular endothelial growth factors VEGF-A and VEGF-C are the main angiogenic factors that control growth of new blood and lymphatic vessels in the organism, and they also possess several immunoregulatory activities. Expression of VEGF-A and VEGF-C mRNA as well as mRNA for VEGF receptors in lymphocytes and macrophages of naive mice was investigated. Using reverse transcription and subsequent polymerase chain reaction, we found that peritoneal macrophages, thymocytes, and lymph node cells constitutively expressed VEGF-A and VEGF-C mRNA. In addition, macrophages were positive for VEGFR-1, VEGFR-2, VEGFR-3, NRP-1, and NRP-2 mRNA, whereas thymocytes and lymph node cells expressed mRNA of the same receptors except VEGFR-1. These data expand our knowledge concerning gene distribution of VEGF receptors in the organism, in particular, among the cells of the immune system. This suggests that, along with their major angiogenic properties, VEGF family members additionally might also perform important mediatory functions within the immune system.

Key words

VEGF-A VEGF-C receptors of VEGF angiogenesis NRP-1 NRP-2 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Ferrara, N., Gerber, H.-P., and LeCouter, J. (2003) Nature Medicine, 6, 669–676.CrossRefGoogle Scholar
  2. 2.
    Barleon, B., Sozzani, S., Zhou, D., Weich, H. A., Mantovani, A., and Marme, D. (1996) Blood, 87, 3336–3343.PubMedGoogle Scholar
  3. 3.
    Mor, F., Quintana, F. J., and Cohen, I. R. (2004) J. Immunol., 172, 4618–4623.PubMedGoogle Scholar
  4. 4.
    Gabrilovich, D., Ishida, T., Oyama, T., Ran, S., Kravtsov, V., Nadaf, S., and Carbone, D. P. (1998) Blood, 92, 4150–4166.PubMedGoogle Scholar
  5. 5.
    Huang, Y., Chen, X., Dikov, M. M., Novitskiy, S. V., Mosse, A., Yang, L., and Carbone, D. P. (2007) Blood, 110, 624–631.PubMedCrossRefGoogle Scholar
  6. 6.
    Crowther, M., Brown, N. J., Bishop, E. T., and Lewis, C. E. (2001) J. Leukoc. Biol., 70, 478–490.PubMedGoogle Scholar
  7. 7.
    Maruyama, K., Masaaki, I., Cursiefen, C., Jackson, D. G., Keino, H., Torrita, M., van Rooijen, N., Takeriaka, H., D’Amore, P. A., Stein-Streilein, J., Losordo, D. W., and Streilein, J. W. (2005) J. Clin. Invest., 115, 2363–2372.PubMedCrossRefGoogle Scholar
  8. 8.
    Owen, L., Iragavarapu-Charyulu, V., Gunja-Smith, Z., Herbert, L. M., Grosso, J. F., and Lopez, D. M. (2003) J. Immunol., 171, 4340–4351.PubMedGoogle Scholar
  9. 9.
    Ijima, K., Yoshikawa, N., and Nakamura, H. (1996) J. Immun. Meth., 196, 199–209.CrossRefGoogle Scholar
  10. 10.
    Partanen, T. A., Arola, J., Saaristo, A., Jussila, L., Ora, A., Miettinen, M., Stacker, S. A., Achen, M. G., and Alitalo, K. (2000) FASEB J., 14, 2087–2096.PubMedCrossRefGoogle Scholar
  11. 11.
    Hiratsuka, S., Minowa, O., Kuno, J., Noda, T., and Shibuta, M. (1998) Proc. Natl. Acad. Sci. USA, 95, 9349–9354.PubMedCrossRefGoogle Scholar
  12. 12.
    McLaren, J., Prentice, A., Charnock-Jones, D. S., Millican, S. A., Muller, K. H., Sharkey, A. M., and Smith, S. K. (1996) J. Clin. Invest., 98, 482–489.PubMedCrossRefGoogle Scholar
  13. 13.
    Yang, Z. F., Poon, R. T., Luo, Y., Cheung, C. K., Ho, D. W., Lo, C. M., and Fan, S. T. (2004) J. Immunol., 173, 2507–2515.PubMedGoogle Scholar
  14. 14.
    Skobe, M., Hamberg, L. M., Hawighorst, T., Schirner, M., Wolf, G. L., Alitalo, K., and Detmar, M. (2001) Am. J. Pathol., 159, 893–903.PubMedGoogle Scholar
  15. 15.
    Wey, J. S., Fan, F., Gray, M. J., Bauer, T. W., McCarty, M. F., Somcio, R., Lui, W., Evans, D. B., Wu, Y., Hicklin, D. J., and Ellis, L. M. (2005) Cancer, 104, 427–438.PubMedCrossRefGoogle Scholar
  16. 16.
    Gray, M. J., Wey, J. S., Belcheva, A., McCarty, M. F., Trevino, J. G., Evans, D. B., Ellis, L. M., and Gallick, G. E. (2005) Cancer Res., 65, 3664–3670.PubMedCrossRefGoogle Scholar
  17. 17.
    Zhukova, L. G., Zhukov, N. V., and Lichinitser, M. R. (2003) Byul. Eksp. Biol. Med., 135, 562–565.Google Scholar
  18. 18.
    Shcherbakov, A. M., Gershtein, E. S., Anurova, O. A., and Kushlinskii, N. E. (2005) Vopr. Onkol., 51, 317–321.PubMedGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2007

Authors and Affiliations

  • O. I. Stepanova
    • 1
  • A. V. Krylov
    • 1
  • V. I. Lioudyno
    • 1
  • E. P. Kisseleva
    • 1
  1. 1.Institute of Experimental MedicineRussian Academy of Medical SciencesSt. PetersburgRussia

Personalised recommendations