Abstract
In this study, we have cloned the Chlamydia trachomatis genes incB and incC into the expression plasmid vectors from pET series for the subsequent isolation of recombinant proteins. As a result, we have obtained the first full-length recombinant C. trachomatis proteins IncB and IncC, which can be used for following antibody production and for study of their protein-protein interaction.
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Weinstock, H., Dean, D., and Bolan, G. (1994) Infect. Dis. Clin. North Am., 8, 797–819.
Stamm, W. E. (1999) J. Infect. Dis., 179, 380–383.
Moulder, J. W. (1991) Microbiol. Rev., 55, 143–190.
Beatty, W. L., Morrison, R. P., and Byrne, G. I. (1994) Microbiol. Rev., 58, 686–699.
Greene, W., Xiao, Y., Huang, Y., McClarty, G., and Zhong, G. (2004) Infect. Immun., 72, 451–460.
Fischer, S. F., Vier, J., Kirschnek, S., Klos, A., Hess, S., Ying, S., and Hacker, G. (2004) J. Exp. Med., 4, 905–916.
Rockey, D., Scidmore, M., Bannantine, J., and Brown, W. (2002) Microbes Infect., 4, 333–340.
Bannantine, J., Griffiths, R., Viratyosin, W., Brown, W., and Rockey, D. (2000) Cell. Microbiol., 2, 35–47.
Toh, H., Miura, K., Shirai, M., and Hattori, M. (2003) DNA Res., 10, 9–17.
Hackstadt, T., Scidmore-Carlson, M. A., Shaw, E. I., and Fischer, E. R. (1999) Cell. Microbiol., 1, 119–130.
Scidmore, M. A., and Hackstadt, T. (2001) Mol. Microbiol., 39, 1638–1650.
Bannantine, J., Rockey, D. D., and Hackstadt, T. (1998) Mol. Microbiol., 28, 1017–1026.
Scidmore-Carlson, M. A., Shaw, E. I., Dooley, C. A., Fischer, E. R., and Hackstadt, T. (1999) Mol. Microbiol., 33, 753–765.
Subtil, A., Parsot, C., and Dautry-Varsat, A. (2001) Mol. Microbiol., 39, 792–800.
Shirai, M., Hirakawa, H., Kimoto, M., Tabuchi, M., Kishi, F., Ouchi, K., Shiba, T., Ishii, K., Hattori, M., Kuhara, S., and Nakazawa, T. (2000) Nucleic Acids Res., 28, 2311–2314.
Read, T., Brunham, R., Shen, C., Gill, S., Heidelberg, J., White, O., Hickey, E., Peterson, J., Utterback, T., Berry, K., Bass, S., Linher, K., Weidman, J., Khouri, H., Craven, B., Bowman, C., Dodson, R., Gwinn, M., Nelson, W., DeBoy, R., Kolonay, J., McClarty, G., Salzberg, S., Eisen, J., and Fraser, C. (2000) Nucleic Acids Res., 28, 1397–1406.
Thomson, N. R., Yeats, C., Bell, K., Holden, M. T. G., Bentley, S. D., Livingstone, M., Cerdeno-Tarraga, A. M., Harris, B., Doggett, J., Ormond, D., Mungal, K., Clarke, K., Feltwell, T., Hance, Z., Sanders, M., Quail, M. A., Price, C., Parkhill, J., and Longbottom, D. (2005) Genome Res., 15, 629–640.
Nicholson, T. L., Olinger, L., Chong, K., Schoolnik, G., and Stephens, R. S. (2003) J. Bacteriol., 185, 3179–3189.
Shaw, E. I., Dooley, C. A., Fischer, E. R., Scidmore, M. A., Fields, K. A., and Hackstadt, T. (2000) Mol. Microbiol., 37, 913–925.
Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: a Laboratory Manual, 2nd Edn., Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.
Hanahan, D. G. (1983) J. Mol. Biol., 5, 557–580.
Rockey, D. D., Viratyosin, W., Bannantine, J. P., Suchland, R. J., and Stamm, W. E. (2002) Microbiology, 148, 2497–2505.
Stewart, E. J., Aslund, F., and Beckwith, J. (1998) EMBO J., 17, 5543–5550.
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Original Russian Text © E. S. Kostrjukova, V. N. Lazarev, G. A. Titova, T. A. Akopian, S. A. Levitskii, V. M. Govorun, 2006, published in Biokhimiya, 2006, Vol. 71, No. 3, pp. 333–341.
Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM05-186, November 13, 2005.
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Kostrjukova, E.S., Lazarev, V.N., Titova, G.A. et al. Expression of Chlamydia trachomatis inclusion membrane protein genes IncB and IncC in Escherichia coli . Biochemistry (Moscow) 71, 262–269 (2006). https://doi.org/10.1134/S0006297906030059
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DOI: https://doi.org/10.1134/S0006297906030059