Biochemistry (Moscow)

, Volume 71, Issue 3, pp 239–244

Effects of vitamin E against aluminum neurotoxicity in rats

  • V. S. Nedzvetsky
  • M. Tuzcu
  • A. Yasar
  • A. A. Tikhomirov
  • G. Baydas


The present study examined the protective effects of vitamin E against aluminum-induced neurotoxicity in rats. Wistar rats were given daily aluminum via their drinking water containing 1600 mg/liter aluminum chloride for six weeks. Aluminum induced a significant increase in lipid peroxidation (LPO) in hippocampus and frontal cortex. Furthermore, aluminum caused marked elevation in the levels of the glial markers (glial fibrillary acidic protein (GFAP) and S100B) and proinflammatory cytokines (TNF-α and IL-1β) in both brain areas. Vitamin E treatment reduced the contents of glial markers and cytokines and the levels of LPO. In conclusion, this study demonstrates that vitamin E ameliorates glial activation and reduces release of proinflammatory cytokines induced by aluminum.

Key words

aluminum vitamin E glial fibrillary acidic protein S100B protein lipid peroxidation glutathione 



lipid peroxidation


reactive oxygen species


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  1. 1.
    Alfrey, A. C., LeGendre, G. R., and Kaehny, W. D. (1976) N. Engl. J. Med., 294, 184–188.PubMedGoogle Scholar
  2. 2.
    Good, P. F., Perl, D. P., Bierer, L. M., and Schmeidler, J. (1992) Ann. Neurol., 31, 286–292.CrossRefPubMedGoogle Scholar
  3. 3.
    Flendrig, J. A., Kruis, H., and Das, H. A. (1976) Lancet, 1, 1235.PubMedGoogle Scholar
  4. 4.
    Kurland, L. T. (1988) Trends Neurosci., 11, 51–58.CrossRefPubMedGoogle Scholar
  5. 5.
    Fu, H. J., Hu, Q. S., Lin, Z. N., Ren, T. L., Song, H., Cai, C. K., and Dong, S. Z. (2003) Brain Res., 980, 11–23.CrossRefPubMedGoogle Scholar
  6. 6.
    Oteiza, P. I., Keen, C. L., Han, B., and Golub, M. S. (1993) Metabolism, 42, 1296–1300.CrossRefPubMedGoogle Scholar
  7. 7.
    Fraga, C. G., Oteiza, P. L., Golub, M. S., Gershwin, M. E., and Keen, C. L. (1990) Toxicol. Lett., 51, 213–219.CrossRefPubMedGoogle Scholar
  8. 8.
    Yousef, M. I. (2004) Toxicology, 199, 47–57.CrossRefPubMedGoogle Scholar
  9. 9.
    Griffin, W. S., Stanley, L. C., Ling, C., White, L., MacLeod, V., Perrot, L. J., White, C. L., and Araoz, C. (1989) Proc. Natl. Acad. Sci. USA, 86, 7611–7615.PubMedGoogle Scholar
  10. 10.
    Suarez-Fernandez, M. B., Soldado, A. B., Sanz-Medel, A., Vega, J. A., Novelli, A., and Fernandez-Sanchez, M. T. (1999) Brain Res., 835, 125–136.PubMedGoogle Scholar
  11. 11.
    Murakami, K., and Yoshino, M. (2004) J. Cell. Biochem., 93, 1267–1271.CrossRefPubMedGoogle Scholar
  12. 12.
    Norton, W. T., Aquino, D. A., Hozumi, I., Chiu, F., and Brosnan, C. F. (1992) Neurochem. Res., 17, 877–885.CrossRefPubMedGoogle Scholar
  13. 13.
    Halliwell, B. (1996) Annu. Rev. Nutr., 16, 33–50.CrossRefPubMedGoogle Scholar
  14. 14.
    Niki, E., Yamamoto, Y., Komuro, E., and Sato, K. (1991) Am. J. Clin. Nutr., 53, 201–205.Google Scholar
  15. 15.
    Sano, M., Ernesto, C., Thomas, R. G., Klauber, M. R., Schafer, K., Grundman, M., Woodbury, P., Growdon, J., Cotman, C. W., Pfeiffer, E., Schneider, L. S., and Thal, L. J. (1997) N. Engl. J. Med., 336, 1216–1222.CrossRefPubMedGoogle Scholar
  16. 16.
    Butterfield, D. A., Koppal, T., Subramaniam, R., and Yatin, S. (1999) Rev. Neurosci., 10, 141–149.PubMedGoogle Scholar
  17. 17.
    Behl, C. (1999) Int. J. Vitam. Nutr. Res., 69, 213–219.PubMedGoogle Scholar
  18. 18.
    Li, Y., Liu, L., Barger, S. W., Mrak, R. E., and Griffin, W. S. (2001) J. Neurosci. Res., 66, 163–170.CrossRefPubMedGoogle Scholar
  19. 19.
    Gong, Q. H., Wu, Q., Huang, X. N., Sun, A. S., and Shi, J. S. (2005) Life Sci., 77, 140–148.CrossRefPubMedGoogle Scholar
  20. 20.
    Lowry, O. H., Rosebrough, N. J., Farr, A. I., and Randall, R. J. (1951) J. Biol. Chem., 193, 265–275.PubMedGoogle Scholar
  21. 21.
    Laemmli, U. K. (1970) Nature, 227, 680–685.CrossRefPubMedGoogle Scholar
  22. 22.
    Nguyen, K. T., Deak, T., Owens, S. M., Kohno, T., Fleshner, M., Watkins, L. R., and Maier, S. F. (1998) J. Neurosci., 18, 2239–2246.PubMedGoogle Scholar
  23. 23.
    Buege, J. A., and Aust, S. D. (1978) Meth. Enzymol., 52, 302–310.PubMedGoogle Scholar
  24. 24.
    Csovari, S., Andyal, T., and Strenger, J. (1991) Lab. Delo, 10, 9–13.PubMedGoogle Scholar
  25. 25.
    Yokel, R. A. (2000) Neurotoxicology, 21, 813–828.PubMedGoogle Scholar
  26. 26.
    Gomez, M., Esparza, J. L., Nogues, M. R., Giralt, M., Cabre, M., and Domingo, J. L. (2005) Free Rad. Biol. Med., 38, 104–111.CrossRefPubMedGoogle Scholar
  27. 27.
    Esparza, J. L., Gomez, M., Romeu, M., Mulero, M., Sanchez, D. J., Mallol, J., and Domingo, J. L. (2003) J. Pineal Res., 35, 32–39.CrossRefPubMedGoogle Scholar
  28. 28.
    Anane, R., and Creppy, E. E. (2001) Hum. Exp. Toxicol., 20, 477–481.CrossRefPubMedGoogle Scholar
  29. 29.
    Dua, R., and Gill, K. D. (2001) Pharmacol. Toxicol., 89, 315–319.CrossRefPubMedGoogle Scholar
  30. 30.
    Abubakar, M. G., Taylor, A., and Ferns, G. A. A. (2003) Int. J. Exp. Pathol., 84, 49–54.CrossRefPubMedGoogle Scholar
  31. 31.
    Exley, C. (2004) Free Rad. Biol. Med., 36, 380–387.CrossRefPubMedGoogle Scholar
  32. 32.
    Azzi, A., Gysin, R., Kempna, P., Ricciarelli, R., Villacorta, L., Visarius, T., and Zingg, J. M. (2003) Mol. Aspects Med., 24, 325–336.PubMedGoogle Scholar
  33. 33.
    Baydas, G., and Canatan, H. (2001) Res. Commun. Pharmacol. Toxicol., 6, 287–292.Google Scholar
  34. 34.
    Baydas, G., Canatan, H., and Turkoglu, A. (2002) J. Pineal Res., 32, 225–230.CrossRefPubMedGoogle Scholar
  35. 35.
    Davis, E. J., Foster, T. D., and Thomas, W. E. (1994) Brain Res. Bull., 34, 73–78.CrossRefPubMedGoogle Scholar
  36. 36.
    Yokel, R. A., and O’Callaghan, J. P. (1998) Neurotoxicol. Teratol., 20, 55–60.CrossRefPubMedGoogle Scholar
  37. 37.
    Platt, B., Fiddler, G., Riedel, G., and Henderson, Z. (2001) Brain Res. Bull., 55, 257–267.CrossRefPubMedGoogle Scholar
  38. 38.
    Guo-Ross, S. X., Yang, E. Y., Walsh, T. J., and Bondy, S. C. (1999) J. Neurochem., 73, 1609–1614.CrossRefPubMedGoogle Scholar
  39. 39.
    Pappolla, M. A., Omar, R. A., Kim, K. S., and Robakis, N. K. (1992) Am. J. Pathol., 140, 621–628.PubMedGoogle Scholar
  40. 40.
    Rothwell, N. J. (1999) J. Physiol., 514, 3–17.CrossRefPubMedGoogle Scholar
  41. 41.
    Kato, H., and Walz, W. (2000) Brain Pathol., 10, 137–143.PubMedGoogle Scholar
  42. 42.
    Viviani, B., Corsini, E., Binaglia, M., Galli, C. L., and Marinovich, M. (2001) Neuroscience, 107, 51–58.CrossRefPubMedGoogle Scholar
  43. 43.
    Chao, C. C., Hu, S., and Peterson, P. K. (1995) Crit. Rev. Neurobiol., 9, 189–205.PubMedGoogle Scholar
  44. 44.
    Chung, I. Y., and Benveniste, E. N. (1990) J. Immunol., 144, 2999–3007.PubMedGoogle Scholar
  45. 45.
    Morganti-Kossman, M. C., Lenzlinger, P. M., Hans, V., Stahel, P., Csuka, E., Ammann, E., Stocker, R., Trentz, O., and Kossmann, T. (1997) Mol. Psychiatry, 2, 133–136.CrossRefPubMedGoogle Scholar
  46. 46.
    Merrill, J. E., and Benveniste, E. N. (1996) Trends Neurosci., 19, 331–338.CrossRefPubMedGoogle Scholar
  47. 47.
    Johnson, V. J., and Sharma, R. P. (2003) Neurotoxicology, 24, 261–268.CrossRefPubMedGoogle Scholar
  48. 48.
    Campbell, A., Becaria, A., Lahiri, D. K., Sharman, K., and Bondy, S. C. (2004) J. Neurosci. Res., 75, 565–572.CrossRefPubMedGoogle Scholar
  49. 49.
    Schreck, R., Rieber, P., and Baeuerle, P. A. (1991) EMBO J., 10, 2247–2258.PubMedGoogle Scholar
  50. 50.
    Baydas, G., Nedzvetskii, V. S., Tuzcu, M., Yasar, A., and Kirichenko, S. V. (2003) Eur. J. Pharmacol., 462, 67–71.CrossRefPubMedGoogle Scholar
  51. 51.
    Meyes, M., Caselman, W. H., Schluter, V., Schreck, R., Hofschneider, P. H., and Baeuerle, P. A. (1992) EMBO J., 11, 2991–3001.Google Scholar

Copyright information

© Pleiades Publishing, Inc. 2006

Authors and Affiliations

  • V. S. Nedzvetsky
    • 1
  • M. Tuzcu
    • 2
  • A. Yasar
    • 3
  • A. A. Tikhomirov
    • 1
  • G. Baydas
    • 3
  1. 1.Department of Biophysics and Biochemistry, Faculty of BiologyDnepropetrovsk National UniversityDnepropetrovskUkraine
  2. 2.Department of Biology, Faculty of ScienceFirat UniversityElazigTurkey
  3. 3.Department of Physiology, Faculty of MedicineFirat UniversityElazigTurkey

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