Skip to main content
SpringerLink
Log in

Study of the Location of Low-Molecular Stress-Inducible Proteins that Protect the Photosynthetic Apparatus against Photodestruction

  • Published:
Applied Biochemistry and Microbiology Aims and scope Submit manuscript

Abstract

An association of low molecular weight, light-inducible HliA/HliA stress proteins with thylakoid chlorophyll–protein complexes of the cyanobacterium Synechocystis sp. PCC 6803 was studied. It is shown via two-dimensional electrophoresis in PAAG, mass spectrometry, and Western blotting that these light-inducible HliA/HliB stress proteins are associated with monomers and trimeric photosystem I (PSI) сomplexes and the photosystem II (PSII) complex. This suggests that these proteins play a universal role in the protection of the photosynthetic apparatus from excess light. To evaluate the functions of Hli proteins and their effect on the photochemical activity of PSI, non-HliA/HliB cells were compared with cyanobacterial cells containing these proteins. It is shown that the PSI photochemical activity was three to four times lower in the absence of HliA/HliB proteins than in the presence of Hli proteins. This suggests that Hli proteins are important for the reaction to oxygen consumption in PSI with an artificial donor and acceptor, which indicates their role in the maintenance of optimal PSI activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.

Similar content being viewed by others

REFERENCES

  1. Karapetyan, N.V., Biochemistry (Moscow), 2007, vol. 72, no. 10, pp. 1127–1135.

    CAS  PubMed  Google Scholar 

  2. Niyogi, K.K., Annu. Rev. Plant Physiol. Plant Mol. Biol., 1999, vol. 50, pp. 333–359.

    Article  CAS  PubMed  Google Scholar 

  3. Kirilovsky, D. and Kerfeld, C.A., Biochim. Biophys. Acta, 2012, vol. 1817, no. 1, p. 158.

    Article  CAS  PubMed  Google Scholar 

  4. Tibiletti, T., Hernández-Prieto, M.A., Matthijs, H.C.P., Niyogi, K.K., and Funk, C., Biochim. Biophys. Acta, 2016, vol. 1857, no. 4, pp. 396–407.

    Article  CAS  PubMed  Google Scholar 

  5. Tibiletti, T., Rehman, A.U., and Vass, I., Photosynth. Res., 2018, vol. 135, nos. 1–3, pp. 103–114.

    Article  CAS  PubMed  Google Scholar 

  6. Beck, J., Lohscheider, J.N., Albert, S., Andersson, U., Mendgen, K.W., Rojas-Stutz, M.C., Adamska, I., and Funck, D., Front. Plant Sci., 2017, vol. 8. https://doi.org/. doi https://doi.org/10.3389/fpls.2017.00007

  7. Funk, C. and Vermaas, W.F.J., Biochemistry, 1999, vol. 38, no. 29, pp. 9397–9404.

    Article  CAS  PubMed  Google Scholar 

  8. Sobotka, R., McLean, S., Zuberova, M., Hunter, C.N., and Tichy, M., J. Bacteriol., 2008, vol. 190, no. 6, pp. 2086–2095.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Sobotka, R., Tichy, M., Wilde, A., and Hunter, C.N., Plant Physiol., 2011, vol. 155, no. 4, pp. 1735–1747.

    Article  CAS  PubMed  Google Scholar 

  10. Storm, P., Tibiletti, T., Hall, M., and Funk, C., PLoS One, 2013, vol. 8. e55569.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Havaux, M., Guedeney, G., He, Q., and Grossman, A.R., Biochim. Biophys. Acta, 2003, vol. 1557, nos. 1–3, pp. 21–33.

    Article  CAS  PubMed  Google Scholar 

  12. Knoppová, J., Sobotka, R., Tichy, M., Yu, J., Konik, P., Halada, P., Nixon, P.J., and Komenda, J., Plant Cell, 2014, vol. 26, no. 3, pp. 1200–1212.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Hernández-Prieto, M.A., Tibiletti, T., Abasova, L., Kirilovsky, D., Vass, I., and Funk, C., Biochim. Biophy-s. Acta, 2011, vol. 1807, no. 9, pp. 1043–1151.

    Google Scholar 

  14. Yao, D.C., Brune, D.C., Vavilin, D., and Vermaas, W.F., J. Biol. Chem., 2012, vol. 287, no. 1, pp. 682–692.

    Article  CAS  PubMed  Google Scholar 

  15. Xu, H., Vavilin, D., Funk, C., and Vermaas, W.F.J., Plant. Mol. Biol., 2002, vol. 49, no. 2, pp. 149–160.

    Article  CAS  PubMed  Google Scholar 

  16. Xu, H., Vavilin, D., Funk, C., and Vermaas, W.F.J., J. Biol. Chem., 2004, vol. 279, no. 27, pp. 27971–27979.

    Article  CAS  PubMed  Google Scholar 

  17. Yurina, N.P., Mokerova, D.V., and Odintsova, M.S., Russ. J. Plant Physiol., 2013, vol. 60, no. 5, pp. 577–588.

    Article  CAS  Google Scholar 

  18. Wang, Q., Jantaro, S., Lu, B., Majeed, W., Bailey, M., and He, Q., Plant Physiol., 2008, vol. 147, no. 3, pp. 1239–1250.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Shen, G., Boussiba, S., and Vermaas, W.F.J., Plant Cell, 1993, vol. 5, no. 12, pp. 1853–1863.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Akulinkina, D.V., Bolychevtseva, Y.V., Elanskaya, I.V., Karapetyan, N.V., and Yurina, N.P., Biochemistry (Moscow), 2015, vol. 80, no. 10, pp. 1254–1261.

    CAS  PubMed  Google Scholar 

  21. Lichtenthaler, H.K., Methods Enzymol., 1987, vol. 148, pp. 350–382.

    Article  CAS  Google Scholar 

  22. Schreiber, U., Klughammer, C., and Neubauer, C., Z. Naturforsch, 1988, vol. 43, pp. 686–698.

  23. Promnares, K., Komenda, J., Bumba, L., Nebesarova, J., Vacha, F., and Tichy, M., J. Biol. Chem., 2006, vol. 281, no. 43, pp. 32705–32713.

    Article  CAS  PubMed  Google Scholar 

  24. Yao, D.C., Kieselbach, T., Komenda, J., Promnares, K., Hernandez-Prieto, M.A., Tichy, M., Vermaas, W.F.J., and Funk, C., J. Biol. Chem., 2007, vol. 282, no. 1, pp. 267–276.

    Article  CAS  PubMed  Google Scholar 

  25. Kufryk, G., Hernandez-Prieto, M.A., Kieselbach, T., Miranda, H., Vermaas, W.F.J., and Funk, C., Photosynth. Res., 2008, vol. 95, nos. 2–3, pp. 135–145.

    Article  CAS  PubMed  Google Scholar 

  26. Shi, L.X., Hall, M., Funk, C., and Schoder, W.P., B-iochim. Biophys. Acta, 2012, vol. 1817, no. 1, pp. 13–25.

    Article  CAS  Google Scholar 

  27. Daddy, S., Zhang, J., Jantara, S., He, C., He, Q., and Wang, Q., Sci. Rep., 2015, vol. 5, p. 9480. doi https://doi.org/10.1038/srep09480

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Kopecna, J., Komenda, J., Bucinska, L., and Sobotka, R., Plant Physiol., 2012, vol. 160, no. 4, pp. 2239–2250.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. Andersson, U., Heddad, M., and Adamska, I., Plant Physiol., 2003, vol. 132, no. 2, pp. 811–820.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Rakhimberdieva, M.G., Boichenko, V.A., Karapetyan, N.V., and Stadnichuk, I.N., Biochemistry, 2001, vol. 40, no. 51, pp. 15780–15788.

    Article  CAS  PubMed  Google Scholar 

  31. El-Mohsnawy, E., Kopczak, M.J., Schlodder, E., Nowaczyk, M., Meyer, H.E., Warscheid, B., Karapetyan, N.V., and Rogner, M., Biochemistry, 2010, vol. 49, no. 23, pp. 4740–4751.

    Article  CAS  PubMed  Google Scholar 

Download references

ACKNOWLEDGMENTS

This work was partly supported by the Russian Foundation for Basic Research, project no. 16-04-01626a, the Presidium of the Russian Academy of Sciences No. I.18 “Molecular and Cellular Biology and Post-Genomic Technologies,” and the FIT Biotechnology, Russian Academy of Sciences, no. 01201351375.

The authors thank V.F.D. Vermaas (University of Arizona, United States) for providing the mutant cyanobacteria.

Author information

Authors and Affiliations

  1. Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences, 119071, Moscow, Russia

    L. S. Sharapova, D. V. Akulinkina, Yu. V. Bolychevseva & N. P. Yurina

  2. Lomonosov Moscow State University, Biology Department, 119991, Moscow, Russia

    I. V. Elanskaya

Authors
  1. L. S. Sharapova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. V. Akulinkina
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yu. V. Bolychevseva
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. I. V. Elanskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. N. P. Yurina
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to I. V. Elanskaya or N. P. Yurina.

Ethics declarations

The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sharapova, L.S., Akulinkina, D.V., Bolychevseva, Y.V. et al. Study of the Location of Low-Molecular Stress-Inducible Proteins that Protect the Photosynthetic Apparatus against Photodestruction. Appl Biochem Microbiol 55, 52–58 (2019). https://doi.org/10.1134/S0003683819010150

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0003683819010150

Keywords:

Search

Navigation