Applied Biochemistry and Microbiology

, Volume 54, Issue 6, pp 603–610 | Cite as

Exogenous and Endogenous Neuroactive Biogenic Amines: Studies with Lactococcus lactis subsp. lactis

  • I. R. VodolazovEmail author
  • S. D. Dbar
  • A. V. Oleskin
  • L. G. Stoyanova


This work demonstrates that Lactococcus lactis subsp. lactis strains that display antimicrobial activity with respect to test cultures (potential pathogens) synthesize biogenic amines. Strains K-205 and F-116 produce submicromolar amounts of dopamine and its 2,3-dihydroxyphenylalanine precursor (DOPA) in a medium that is devoid of dopamine and DOPA. It was also revealed that exogenous neuromediators such as adrenaline (epinephrine), dopamine, and serotonin stimulated the growth and antimicrobial activity of some strains at micromolar concentrations. The growth of L. lactis subsp. lactis strain 194 was threefold accelerated by all the neurotransmitters. The stimulatory effect was observed after 6 and 12 h of cultivation. Epinephrine and serotonin failed to produce a statistically significant effect on the growth of the other tested strains (K-205, 729, and F-116). Among the neurochemicals under study, dopamine exerted the maximum (40%) stimulatory effect on the antimicrobial activity of the tested strains with both gram-positive and negative bacteria. However, these strains only insignificantly influenced the growth of fungi, presumably because this process was activated by the neurochemicals.


Lactococcus lactis subsp. lactis antimicrobial activity potential pathogens neuromediators (neurochemicals) epinephrine (adrenaline) dopamine serotonin DOPA 


  1. 1.
    Nuryshev, M.Zh., Stoyanova, L.G., and Netrusov, I.A., J. Microb. Biochem. Technol., 2016, vol. 8, no. 4, pp. 290–295.CrossRefGoogle Scholar
  2. 2.
    El Aidy, S, Dinan, T.G and Cryan, J.F., Clin. Therapy, 2015, vol. 37, pp. 954–967.CrossRefGoogle Scholar
  3. 3.
    Abbott, A., Nature, 2016, vol. 531, pp. 294–297.CrossRefGoogle Scholar
  4. 4.
    Shenderov, B.A., Metamorfozy, 2014, no. 5, pp. 72–80.Google Scholar
  5. 5.
    Krishnan, S., Alden, N. and Lee, K., Curr. Opin. Biotech., 2015, vol. 36, pp. 137–145.CrossRefGoogle Scholar
  6. 6.
    Li, Q. and Zhou, J.M., Neuroscience, 2016, vol. 324, pp. 131–139.CrossRefGoogle Scholar
  7. 7.
    Kelly, J.R., Clarke, G., Cryan, J.F. and Dinan, T.G., Ann. Epidemiol., 2016, vol. 26, no. 5, pp. 366–372.CrossRefGoogle Scholar
  8. 8.
    Oleskin, A.V., El’–Registan, G.I. and Shenderov, B.A., Microbiology, 2016, vol. 85, no. 1, pp. 1–22.CrossRefGoogle Scholar
  9. 9.
    Lyte, M., Adv. Exp. Med. Biol., 2016, vol. 874, pp. 1–24.Google Scholar
  10. 10.
    Oleskin, A.V., Shenderov, B.A. and Rogovsky, V.S., Probiotics Antimicrob. Proteins, 2017, vol. 9, no. 3, pp. 215–234.CrossRefGoogle Scholar
  11. 11.
    Oleskin, A.V, Zhilenkova, O.G., Shenderov, B.A., Amerhanova, A.M., Kudrin, V.S., Klodt, P.M., J. Pharm. Nutrit. Sci., 2014, vol. 4, pp. 199–206.CrossRefGoogle Scholar
  12. 12.
    Stoyanova, L.G., Sul’timova, T.D., Botina, S.G. and Netrusov, A.I., Appl. Biochem. Microbiol., 2006, vol. 42, no. 5, pp. 492–499.CrossRefGoogle Scholar
  13. 13.
    Shpakov, A.O., Microbiology, 2009, vol. 78, no. 2, pp. 163–175.Google Scholar
  14. 14.
    Yunes, R.A., Poluektova, R.U., Dyachkova, M.S., Klimina, K.M., Kovtun, A.S., Averina, O.V., Orlova, V.S. and Danilenko, V.N., Anaerobe, 2016, vol. 42, pp. 1–8.CrossRefGoogle Scholar
  15. 15.
    Oleskin, A.V., Kirovskaya, T.A., Botvinko, I.V. and Lysak, L.V., Microbiology, 1998, vol. 67, no. 3, pp. 306–311.Google Scholar
  16. 16.
    Anuchin, A.M., Chuvelev, D.I., Kirovskaya, T.A. and Oleskin, A.V., Microbiology, 2008, vol. 77, no. 6, pp. 758–765.CrossRefGoogle Scholar
  17. 17.
    Malikina, K.D., Shishov, V.A., Chuvelev, D.I., Kudrin, V.S. and Oleskin, A.V., Appl. Biochem. Microbiol., 2010, vol. 46, no. 6, pp. 672–677.CrossRefGoogle Scholar
  18. 18.
    Oleskin, A.V., Shishov, V.I., and Malikina, K.D., Symbiotic Biofilms and Brain Neurochemistry, Hauppauge. N.Y.: Nova Science Publishers, 2010.Google Scholar
  19. 19.
    Eldrup, E.D., Med. Bull., 2004, vol. 31, pp. 34–62.Google Scholar
  20. 20.
    McPherson, R.A. and Pincus, M.R., Henry’s Clinical Diagnosis and Management by Laboratory Methods, Philadelphia: Elsevier Saunders, 2011.Google Scholar
  21. 21.
    Dubynin, V.A., Kamenskii, A.A., Sapin, M.R., and Sivoglazov, V.N., Regulyatornye sistemy organizma cheloveka (Regulatory Systems of the Human Organism), Moscow: Drofa, 2003.Google Scholar

Copyright information

© Pleiades Publishing, Inc. 2018

Authors and Affiliations

  • I. R. Vodolazov
    • 1
  • S. D. Dbar
    • 1
  • A. V. Oleskin
    • 1
  • L. G. Stoyanova
    • 1
  1. 1.Lomonosov Moscow State University, Biological FacultyMoscowRussia

Personalised recommendations