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Applied Biochemistry and Microbiology

, Volume 54, Issue 6, pp 682–688 | Cite as

Taqman RT-PCR Analysis for the Identification of the Osmotolerant Yeast Zygosaccharomyces rouxii

  • M. Yu. SyromyatnikovEmail author
  • O. V. Savinkova
  • S. V. Kiryanova
  • E. N. Frolov
  • V. N. Popov
Article
  • 18 Downloads

Abstract

A rapid method for the identification of the osmotolerant yeast Zygosaccharomyces rouxii was developed based on Taqman RT-PCR. Osmotolerant yeasts cause spoilage of food with a high sugar content (honey, syrups, candies, jams, concentrated fruit juices, etc.). In the course of bioinformatics analysis, a DNA segment containing the 5.8S, 18S and 28S genes of ribosomal RNA and ITS1 and ITS2 intergenic regions specific for Z. rouxii was detected, and primers and Taqman probes were developed. The PCR was optimized both for the concentration of its components and for temperature cycles. It was shown that PCR is specific only for Z. rouxii, and the developed analysis allowed the detection of 10 femtograms of its DNA. The method was validated for the collection microorganisms of different taxonomic groups. As a result of the screening of 22 commercially available sugars and dried fruits, it was established that three types of sugar and six dried fruits were contaminated with these yeasts.

Keywords:

Zygosaccharomyces rouxii Taqman RT-PCR identification screening 

Notes

REFERENCES

  1. 1.
    Tilbury, R.H., in Xerotolerant Yeasts at High Sugar Concentration, Skinner, F.A., Passmore, S., and Davenport, R.R., Eds., London: Academic Press, 1980, pp. 153–180.Google Scholar
  2. 2.
    Tilbury, R.H., in Intermediate Moisture Foods, Davies, R., Birch, G.G., and Parker, K.J., Eds., London: Appl. Sci. Publ., 1976, pp. 138–165.Google Scholar
  3. 3.
    Scott, W.J., Adv. Food Res., 1957, vol. 7, pp. 83–127.CrossRefGoogle Scholar
  4. 4.
    Marvig, C.L., Kristiansen, R.M., Madsen, M.G., and Nielsen, D.S., Int. J. Food Microbiol., 2014, vol. 185, no. 2, pp. 167–176.CrossRefGoogle Scholar
  5. 5.
    Splittstoesser, D.F., Fruits and Fruit Products. Food and Beverage Mycology, Beuchat, L.R., Ed., Westport, CT: AVI Publ. Co., 1978, pp. 83–109.Google Scholar
  6. 6.
    Martorell, P., Fernandez-Espinar, M.T., and Querol, A., Int. J. Food Microbiol., 2005, vol. 101, no. 3, pp. 293–302.CrossRefGoogle Scholar
  7. 7.
    Rojo, M.C., Torres, Palazzolo, C., Cuello, R., González, M., Guevara, F., Ponsone, M.L., Mercado, L.A., Martínez, C., and Combina, M., Food Microbiol., 2017, vol. 64, no. 1, pp. 7–14.CrossRefGoogle Scholar
  8. 8.
    Cho, S.H., Park, H.S., Jo, S.W., Yim, E.J., Yang, H.Y., Ha, G.S., Kim, E.J., Yang, S.J., and Jeong, D.Y., Korean J. Microbiol., 2017, vol. 53, no. 1, pp. 39–48.Google Scholar
  9. 9.
    Bai, J., Yue, T., Wang, H., Niu, C., and Yang, L., Trans. Chin. Soc. Agricult. Eng., 2016, vol. 32, no. 2, pp. 279–284.Google Scholar
  10. 10.
    Solieri, L. and Giudici, P., Int. J. Food Microbiol., 2008, vol. 125, no. 1, pp. 36–45.CrossRefGoogle Scholar
  11. 11.
    Hamad, S.H., J. Food Prot., 2008, vol. 71, no. 7, pp. 1406–1411.CrossRefGoogle Scholar
  12. 12.
    Senses-Ergul, S. and Ozbas, Z.Y., J. Gen. Appl. Microbiol., 2006, vol. 52, no. 2, pp. 99–106.CrossRefGoogle Scholar
  13. 13.
    Onishi, H., Adv. Food Res., 1963, vol. 12, pp. 53–94.CrossRefGoogle Scholar
  14. 14.
    Xu, H., Liu, S.-C., Nie, R., Liu, Y., Li, L.-M., and Wang, F., Modern Food Sci. Technol., 2013, vol. 29, no. 5, pp. 1076–1079.Google Scholar
  15. 15.
    Restaino, L., Bills, S., Tscherneff, K., and Lenovich, L.M., Appl. Environ. Microbiol., 1983, vol. 45, no. 5, pp. 1614–1621.Google Scholar
  16. 16.
    Leandro, MJ., Cabral, S., Prista, C., Loureiro-Dias, M.C., and Sychrova, H., Eukaryotic Cell, 2014, vol. 13, no. 11, pp. 1371–1379.CrossRefGoogle Scholar
  17. 17.
    Dakal, T.C., Solieri, L., and Giudic, P., Int. J. Food Microbiol., 2014, vol. 185, no. 2, pp. 140–157.CrossRefGoogle Scholar
  18. 18.
    Zimmermannova, O., Salazar, A., Sychrova, H., and Ramo, J., FEMS Yeast Res., 2015, vol. 15, no. 4. fov029.CrossRefGoogle Scholar
  19. 19.
    Dušková, M., Ferreira, C., Lucas, C., and Sychrová, H., Mol. Microbiol., 2015, vol. 97, no. 3, pp. 541–559.CrossRefGoogle Scholar
  20. 20.
    Wang, H., Hu, Z., Long, F., Guo, C., Yuan, Y., and Yue, T., Food Microbiol., 2016, vol. 217, no. 1, pp. 68–78.CrossRefGoogle Scholar
  21. 21.
    del Carmen, Jaramillo M., Huttener, M., Manuel, Alvarez J., Homs-Corbera, A., Samitier, J., Torrents, E., and Juarez, A., Electrophoresis, 2015, vol. 36, no. 13, pp. 1471–1478.CrossRefGoogle Scholar
  22. 22.
    Dakal, T.C., Solieri, L., and Giudici, P., Food Microbiol., 2017, vol. 72, no. 2, pp. 135–145.CrossRefGoogle Scholar
  23. 23.
    Casey, G.D. and Dobson, A.D., Int. J. Food Microbiol., 2004, vol. 91, no. 3, pp. 327–335.CrossRefGoogle Scholar
  24. 24.
    Yoshikawa, S., Yasokawa, D., Nagashima, K., Yamazaki, K., Kurihara, H., Ohta, T., and Kawai, Y., Food Microbiol., 2010, vol. 27, no. 4, pp. 509–514.CrossRefGoogle Scholar
  25. 25.
    Senses-Ergul, S., Karasu-Yalcin, S., and Ozbas, Z.Y., Ann. Microbiol., 2012, vol. 62, no. 4, pp. 1501–1516.CrossRefGoogle Scholar
  26. 26.
    Hulin, M. and Wheals, A., Int. J. Food Microbiol., 2014, vol. 173, no. 1, pp. 9–13.CrossRefGoogle Scholar
  27. 27.
    Harrison, E., Muir, A., Stratford, M., and Wheals, A., FEMS Yeast Res., 2011, vol. 11, no. 4, pp. 356–365.CrossRefGoogle Scholar
  28. 28.
    Warth, A.D., J. Appl. Bacteriol., 1977, vol. 43, no. 2, pp. 215–230.CrossRefGoogle Scholar
  29. 29.
    Golden, D.A. and Beuchat, L.R., J. Appl. Bacteriol., 1992, vol. 73, no. 6, pp. 524–530.CrossRefGoogle Scholar
  30. 30.
    Rojo, M.C., Arroyo López, F.N., Lerena, M.C., Mercado, L., Torres, A., and Combina, M., Food Control, 2015, vol. 50, no. 4, pp. 349–355.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Inc. 2018

Authors and Affiliations

  • M. Yu. Syromyatnikov
    • 1
    Email author
  • O. V. Savinkova
    • 1
  • S. V. Kiryanova
    • 3
  • E. N. Frolov
    • 2
  • V. N. Popov
    • 1
  1. 1.Voronezh State UniversityVoronezhRussia
  2. 2.Winogradsky Institute of Microbiology, Fundamentals of Biotechnology Federal Research Center, Russian Academy of SciencesMoscowRussia
  3. 3.Biruch-NT Innovative Center (EFKO)AlekseevkaRussia

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