Applied Biochemistry and Microbiology

, Volume 54, Issue 6, pp 695–701 | Cite as

Method for Evaluation of the Antioxidant Properties of Preparations in Model System Based on Paprika Carotenoids

  • T. A. MisharinaEmail author
  • M. G. Semenova
  • V. I. Kiseleva


A method for the estimation of antioxidant properties of natural and synthetic preparations was developed based on inhibition of the autoxidation of paprika carotenoids. Carotenoids in the form of paprika extract were coated on a porous inert polysaccharide. Autoxidation of paprika carotenoids occurs more rapidly in such system than with direct methods for the determination of antioxidant inhibitory properties. It was shown that the developed method had good reproducibility. The behavior of paprika carotenoids, which was studied in systems with various types of starches, showed that the developed method could be used to evaluate the protective properties of food biopolymers in relation to lipid autoxidation. Moreover, the method is effective for comparison of the activity of both synthetic and natural antioxidants. The most important advantage of the method is that paprika carotenoids are almost identical to unsaturated fatty acids in their ability to interact with oxygen radicals of oxidants. Therefore, the results reflect the behavior of antioxidants in both real lipid-containing model systems and food products.

Kеywords: spectrophotometry antioxidants paprika carotenoids essential oils and spices extracts 



  1. 1.
    Guichard, E., Food Rev. Int., 2002, vol. 18, no. 1, pp. 49–70.CrossRefGoogle Scholar
  2. 2.
    Goubert, I., Quere, J-L., and Voilley, A., J. Agric. Food Chem., 1998, vol. 46, no. 5, pp. 1981–1990.CrossRefGoogle Scholar
  3. 3.
    Misharina, T.A., Appl. Biochem. Microbiol., 2002, vol. 38, no. 5, pp. 480–486.CrossRefGoogle Scholar
  4. 4.
    Misharina, T.A., Appl. Biochem. Microbiol., 2004, vol. 40, no. 1, pp. 104-107.CrossRefGoogle Scholar
  5. 5.
    Misharina, T.A., Terenina, M.B., and Krikunova, N.I., Prikl. Biokhim. Mikrobiol., 2007, vol. 43, no. 6, pp. 678–685.Google Scholar
  6. 6.
    Han, R.-M., Chen, C.-H., Tian, Y.-X., Zhang, J.-P., and Skibsted, L.H., J. Phys. Chem., 2009, vol. 114, no. 1, pp. P. 126–132.Google Scholar
  7. 7.
    Skibsted, L.H., J. Food Drug Analysis, 2012, vol. 20, no. 2, pp. 355–358.Google Scholar
  8. 8.
    Miguel, M.G., Molecules, 2010, vol. 15, no. 9, pp. 9252–9287.CrossRefGoogle Scholar
  9. 9.
    Lissi, E.A., Food Chem., 2005, vol. 92, no. 1, pp. 235–254.CrossRefGoogle Scholar
  10. 10.
    Hornero-Mendes, D. and Minguez-Mosquera, M.I., J. Agric. Food Chem., 2001, vol. 49, no. 8, pp. 3584–3588.CrossRefGoogle Scholar
  11. 11.
    Misharina, T.A., Terenina, M.B., and Krikunova, N.I., Appl. Biochem. Microbiol., 2009, vol. 45, no. 6, pp. 642–647.CrossRefGoogle Scholar
  12. 12.
    Rodrigues, P., Morais, H., Mota, T., Olivera, S., Forgaes, E., and Cserhati, T., Anal. Chim. Acta, 1998, vol. 372, no. 3, pp. 411–416.CrossRefGoogle Scholar
  13. 13.
    Deli, J. and Toth, G., Zeitschrift fur Lebensm. Unters. Forsch., 1997, vol. 205, no. 5, pp. 388–391.CrossRefGoogle Scholar
  14. 14.
    Sarafanova, L.A., Pishchevye dobavki: Entsiklopediya (Nutritional Supplements: Encyclopedia), St. Petersburg: GIOR, 2004, 2nd ed.Google Scholar
  15. 15.
    Golovnya, R.V. and Misharina, T.A., Izv. Akad. Nauk, Ser. Khim., 1998, no. 2, pp. 310–313.Google Scholar
  16. 16.
    Arvisenet, G., Volley, A., and Cayot, N., J. Agric. Food Chem., 2002, vol. 50, no. 25 P, pp. 7345–7349.Google Scholar
  17. 17.
    Gelders, G.G., Goesaert, H., and Delcour, J.A., J. Agric. Food Chem., 2006, vol. 54, no. 6, pp. 1493 –1498.CrossRefGoogle Scholar
  18. 18.
    Wassermann, L.A., Misharina, T.A., and Yuryev, V.P., in Starch and Starch Containing Origins—Structure, Properties and New Technologies, Yuryev, V.P., Cesaro, A., and Berytaller, W.J., Eds., New York: Nova Sci. Publ. Inc., 2002, pp. 63–80.Google Scholar
  19. 19.
    Goubert, I., Quere, J-L., Semon, E., Seuvre, A-M., and Voilley, A., in Flavour Release, Roberts, D.D. and Taylor, A.J., Eds., Washington, DC: Am. Chem. Soc., 2000, pp. 246–259.Google Scholar
  20. 20.
    Karadag, A., Ozcelik, B., and Saner, S., Food Anal. Methods, 2009, vol. 2, no. 1, pp. 41–60.CrossRefGoogle Scholar
  21. 21.
    Handbook of Antioxidants for Food Preservation, Shahidi, F., Ed., Amsterdam: Elsevier, 2015.Google Scholar
  22. 22.
    Chemistry of Spices, Parthasarathy, V.A., Chempakam, B., and Zachariah, T. J., Oxfordshire: CAB Int., 2008, pp. 260–286.Google Scholar
  23. 23.
    Charles, D.J., Antioxidant Properties of Spices, Herbs and Other Sources, New York: Springer, 2013.CrossRefGoogle Scholar
  24. 24.
    Misharina, T.A., Appl. Biochem. Microbiol., 2001, vol. 37, no. 6, pp. 622–628.CrossRefGoogle Scholar
  25. 25.
    Bonde, V., Brand-Williams, W., and Berset, C., Lebensm. Wiss. Technol., 1997, vol. 30, pp. 609–615.CrossRefGoogle Scholar
  26. 26.
    Misharina, T.A., Alinkina, E.S., and Medvedeva, I.B., Appl. Biochem. Microbiol., 2015, vol. 51, no. 1, pp. 119–124.CrossRefGoogle Scholar
  27. 27.
    Misharina, T.A., Alinkina, E.S., Vorob’eva, A.K., Terenina, M.B., and Krikunova, N.I., Appl. Biochem. Microbiol., 2016, vol. 52, no. 3, pp. 336–341.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Inc. 2018

Authors and Affiliations

  • T. A. Misharina
    • 1
    • 2
    Email author
  • M. G. Semenova
    • 1
  • V. I. Kiseleva
    • 1
  1. 1.Emmanuel Institute of Biochemical Physics, Russian Academy of SciencesMoscowRussia
  2. 2.Plekhanov Russian University of EconomicsMoscowRussia

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