Applied Biochemistry and Microbiology

, Volume 54, Issue 6, pp 677–681 | Cite as

Anti-Trinitrotoluene Aptamers: Design, Functional Assessment and Optimization

  • M. Alipour
  • M. ZeinoddiniEmail author
  • A.R. Saeeidinia


The aim of this work is detection of trinitrotoluene (TNT) using aptamer as a new sensing strategy. Two pBluescript plasmids containing RT and ST anti-TNT aptamers were used as templates for aptamer amplification by PCR method. For this purpose, 126 bp ST-aptamer and 118 bp RT-aptamer were amplified using specific primers. TNT bound to bovine serum albumin (TNP-BSA) was used as the antigen, and digoxigenin (DIG)-labeled aptamers were detected by horseradish peroxidase conjugated to anti-DIG monoclonal antibodies. The sensitivity and specificity of ST- and RT-aptamers were determined using optimized PCR and enzyme-linked aptamer-sorbent assay. The sensitivity of this detection after optimization was determined about 1.76 nM of TNT using 1 pM of aptamers. These results indicated favorable functions of both aptamers for TNT detection that can be used in the future as aptasensors for investigation and utilization of the TNT identification.


aptamer trinitrotoluene detection of explosives PCR enzyme-linked aptamer-sorbent assay 


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Copyright information

© Pleiades Publishing, Inc. 2018

Authors and Affiliations

  1. 1.Department of Bioscience and Biotechnology, Malek Ashtar University of TechnologyTehranIran

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