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Applied Biochemistry and Microbiology

, Volume 54, Issue 4, pp 425–431 | Cite as

Preparation and Characterization of Alphitobius diaperinus Melanin

  • N. A. Ushakova
  • A. E. Dontsov
  • N. L. Sakina
  • V. P. Karagodin
  • A. I. Bastrakov
Article
  • 16 Downloads

Abstract

Melanin with a high antioxidant and sorption activity comparable to that of synthetic dioxyphenylalanine (DOPA)-melanin was isolated from the biomass of the darkling beetle Alphitobius diaperinus. The pigment was extracted with a solution of potassium hydroxide, followed by precipitation with concentrated hydrochloric acid and hydrolysis of the resulting precipitate with the same acid. The electron paramagnetic resonance (EPR) signal of melanin was characteristic of eumelanins with a spin concentration of 4.9 × 1017 spin per 1 g of dry weight. The melanin concentration that induced 50% inhibition of peroxidation was 9.2 μg/mL (the analogous concentration of DOPA-melanin was 8.0 μg/mL). The maximum of methylene-blue binding to the beetle melanin was 700 mg of dye per 1 g of dry weight of the preparation. The lipid-free melanin preparation exhibited antiradical activity.

Keywords

Alphitobius diaperinus melanin extraction antioxidant activity sorption capacity EPR signal 

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References

  1. 1.
    Ostrovskii, M.A. and Dontsov, A.E., Fiziol. Chel., 1985, vol. 11, no. 4, pp. 670–678.Google Scholar
  2. 2.
    Baraboi, V.A., Usp. Sovrem. Biol., 2000, no. 117, pp. 86–92.Google Scholar
  3. 3.
    Dontsov, A.E., Zashchitnoe deistvie melaninov pri okislitel’nom stresse (Protective Effect of Melanins in Oxidative Stress), Saarbrücken: Lambert Acad. Publ., 2014.Google Scholar
  4. 4.
    Meredith, P. and Sarna, T., Pigment Cell Res., 2006, vol. 19, pp. 572–594.CrossRefPubMedGoogle Scholar
  5. 5.
    Ushakova, N.A., Koval’zon, V.M., Bastrakov, A.I., Kozlova, A.A., Revishchin, A.V., Pavlova, G.V., and Pavlov, D.S., Dokl. Biochem. Biophys., 2015, vol. 461, pp. 94–97.CrossRefPubMedGoogle Scholar
  6. 6.
    Koval’zon, V.M., Ushakova, N.A., Bastrakov, A.I., Kozlova, A.A., Ambaryan, A.V., Shevchenko, V.P., Nagaev, I.Yu., and Pavlov, D.S., Ross. Fiziol. Zh., 2016, vol. 102, no. 4, pp. 436–441.Google Scholar
  7. 7.
    Smirnov, L.D., Kuznetsov, Yu.V., Proskuryakov, S.Ya., Skvortsov, V.G., Nosko, T.N., and Dontsov, A.E., Biofizika, 2011, vol. 56, no. 2, pp. 316–321.PubMedGoogle Scholar
  8. 8.
    Buege, J.A. and Aust, S.D., Methods in Enzymology, New York: Academic Press, 1978, vol. 52, pp. 302–310.CrossRefPubMedGoogle Scholar
  9. 9.
    Kayatz, P., Thumann, G., Luther, T.T., Jordan, J.F., Bartz-Schmidt, K.U., Esser, P.J., and Schraermeyer, U., Invest. Ophthamol. Vis. Sci., 2001, vol. 42, no. 1, pp. 241–246.Google Scholar
  10. 10.
    Prutenskaya, E.A., Vasil’ev, A.S., Lebedeva, E.Yu., and Sidorov, A.I., Vestn. Tversk. Gos. Tekhn. Univ., 2017, no. 31, pp. 129–132.Google Scholar
  11. 11.
    Zhang, Z.-T., Cao, X.-B., Xiong, N., Wang, H.-C., Huang, J.-S., Sun, S.-G., and Wang, T., Acta Pharmacologica Sinica, 2010, vol. 31, pp. 900–906.CrossRefPubMedPubMedCentralGoogle Scholar
  12. 12.
    Gekht, A.B., Popov, G.R., Gudkova, A.A., Korshunova, E.S., Boldyreva, E.A., and Gusev, E.I., Neirodegenerativnye zabolevaniya: ot genoma do tselostnogo organizma (Neurodegenerative Diseases: From the Genome to the Whole Organism), Ugryumov, M.V., Ed., Moscow: Nauchnyi mir, 2014, vol. 1, pp. 74–95.Google Scholar
  13. 13.
    Nodel’, M.R., Nevrol. Neiropsikhiatr. Psikhosomat., 2013, no. 2, pp. 29–34.Google Scholar

Copyright information

© Pleiades Publishing, Inc. 2018

Authors and Affiliations

  • N. A. Ushakova
    • 1
  • A. E. Dontsov
    • 2
  • N. L. Sakina
    • 2
  • V. P. Karagodin
    • 3
  • A. I. Bastrakov
    • 1
  1. 1.Severtsov Institute of Ecology and EvolutionRussian Academy of SciencesMoscowRussia
  2. 2.Emanuel Institute of Biochemical PhysicsRussian Academy of SciencesMoscowRussia
  3. 3.Plekhanov Russian Economic UniversityMoscowRussia

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