Proteolytic Activity of Chymotrypsin Immobilized on Selenium Nanoparticles
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Chymotrypsin was immobilized as a part of hybrid organo-inorganic nanocomplexes on selenium nanoparticles in the reduction reaction of selenious acid with ascorbic acid. Chymotrypsin stabilized the selenium nanoparticles in the solution. The spectral characteristics of synthesized nanocomplexes and the proteolytic activity of immobilized chymotrypsin were studied. It was shown that the immobilized enzyme had increased proteolytic activity in an alkaline medium.
Keywordsimmobilization of enzymes chymotrypsin selenium nanoparticles
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- 5.Borovikova, L.N., Titova, A.V., Matveeva, N.A., and Pisarev, O.A., Zh. Fiz. Khim., 2013, vol. 87, no. 6, pp. 1008–1011.Google Scholar
- 6.Borovikova, L.N., Titova, A.V., Kipper, A.I., and Pisarev, O.A., Zh. Fiz. Khim., 2015, vol. 89, no. 3, pp. 467–469.Google Scholar
- 7.Varfolomeev, S.D., Khimicheskaya enzimologiya (Chemical Enzymology), Moscow: Izdat. Tsentr Akademiya, 2005.Google Scholar
- 8.Demchenko, A.P., Ul’trafioletovaya spektrofotometriya i struktura belkov (Ultraviolet Spectrophotometry and Protein Structure), Kiev: Naukova dumka, 1981.Google Scholar