Applied Biochemistry and Microbiology

, Volume 54, Issue 2, pp 179–187 | Cite as

Study of the Conditions for the Biosynthesis of Bacterial Cellulose by the Producer Medusomyces gisevii Sa-12

  • E. K. Gladysheva
  • E. A. Skiba
  • V. N. Zolotukhin
  • G. V. Sakovich


The effect of culture conditions on the bacterial cellulose (BC) yield synthesized by symbiotic culture Medusomyces gisevii Sa-12 grown in synthetic nutrient medium was studied for the first time. The conditions providing the maximum yield of bacterial cellulose were evaluated: the initial glucose concentration in the culture medium was 20–25 g/L, the temperature was 24–27°C, the ratio of the nutrient medium to the air volume was 1: 10, and the content of the black tea extracts comprised 1.6–4.8 g/L. A sample of chemically pure BC with the following characteristics was obtained under the selected culture conditions: moisture— 99.0%, degree of polymerization—4800, average width of microfibrillar ribbons—30.6 nm, degree of crystallinity— 86%, and the content of triclinic modification Iα—98%.


bacterial cellulose symbiotic culture cellulose producers culture conditions 


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Copyright information

© Pleiades Publishing, Inc. 2018

Authors and Affiliations

  • E. K. Gladysheva
    • 1
  • E. A. Skiba
    • 1
  • V. N. Zolotukhin
    • 1
  • G. V. Sakovich
    • 1
  1. 1.Institute for Problems of Chemical and Energetic Technologies of the Siberian Branch of the Russian Academy of SciencesBiyskRussia

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