Applied Biochemistry and Microbiology

, Volume 53, Issue 2, pp 258–266 | Cite as

Effect of lipopolysaccharide O-side chains on the adhesiveness of Yersinia pseudotuberculosis to J774 macrophages as revealed by optical tweezers

  • A. A. Byvalov
  • V. L. Kononenko
  • I. V. Konyshev
Article

Abstract

A method has been developed for the quantitative estimation of the binding force of a model microsphere with a eukaryocyte based on the optical trap in order to study the molecular mechanism of adhesion between an individual bacterium and a host cell. The substantial role of LPS O-side chains in the adhesiveness of Yersinia pseudotuberculosis 1b to J774 macrophages has been revealed with the use of a set of microspheres functionalized with lipopolysaccharide (LPS) preparations and antibodies with different specificities. The results indicate the significance of the O-antigen as a pathogenicity factor of Y. pseudotuberculosis in colonization of a macroorganism. The developed methodical approaches can be applied to the study of molecular mechanisms of the pathogenesis of pseudotuberculosis and other infectious diseases to improve antiepidemic service.

Keywords

Yersinia pseudotuberculosis lipopolysaccharide O-side chains monoclonal antibodies optical tweezers microspheres adhesion 

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Copyright information

© Pleiades Publishing, Inc. 2017

Authors and Affiliations

  • A. A. Byvalov
    • 1
    • 2
  • V. L. Kononenko
    • 3
    • 4
  • I. V. Konyshev
    • 1
    • 2
  1. 1.Vyatka State UniversityKirov, ]610000Russia
  2. 2.Institute of Physiology, Komi Research Center, Ural BranchRussian Academy of SciencesSyktyvkarRussia
  3. 3.Emanuel Institute of Biochemical PhysicsRussian Academy of SciencesMoscowRussia
  4. 4.Dmitry Rogachev Federal Research Center of Pediatric Hematology, Oncology, and ImmunologyMoscowRussia

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