Advertisement

Applied Biochemistry and Microbiology

, Volume 52, Issue 1, pp 43–50 | Cite as

The effect of organic solvents on the viability and morphofunctional properties of rhodococcus

  • I. O. Korshunova
  • O. N. Pistsova
  • M. S. Kuyukina
  • I. B. Ivshina
Article

Abstract

The effect of organic solvents on the viability and structural-mechanical (size, surface roughness, and elasticity) properties of actinobacteria of the genus Rhodococcus was studied. The dependence of the survival of Rhodococcus on the hydrophobicity of the solvent, the culture density, and the incubation medium composition was demonstrated. The most resistant strains have been selected. Using combined confocal laser scanning and atomic force microscopy, we established that exposure to solvents resulted in an increase in the relative area and roughness of the surface of living cells and a decrease in their Young’s modulus. The involvement of protonand sodium-dependent efflux pumps in the formation of solvent tolerance in Rhodococcus was demonstrated.

Keywords

actinobacteria Rhodococcus organic solvents resistance atomic force microscopy confocal laser scanning microscopy efflux pumps 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Cabral, J.M.S., Aires-Barros, M.R., Pinheiro, H., and Prazeres, D.M.F., J. Biotechnol., 1997, vol. 59, no. 1–2, pp. 133–143.CrossRefPubMedGoogle Scholar
  2. 2.
    Schmid, A., Dordick, J.S., Hauer, B., Kiener, A., Wubbolts, M., and Witholt, B., Nature, 2001, vol. 409, no. 6817, pp. 258–268.CrossRefPubMedGoogle Scholar
  3. 3.
    Ishige, T., Honda, K., and Shimizu, S., Curr. Opin. Chem. Biol., 2005, vol. 9, no. 2, pp. 174–180.CrossRefPubMedGoogle Scholar
  4. 4.
    Zingaro, K.A., Nicolaou, S.A., and Papoutsakis, E.T., Trends Biotechnol., 2013, vol. 31, no. 11, pp. 643–653.CrossRefPubMedGoogle Scholar
  5. 5.
    Bezborodov, A.M., Zagustina, N.A., and Popov, V.O., Fermentativnye protsessy v biotekhnologii (Enzymatic Processes in Biotechnology), Moscow: Nauka, 2008.Google Scholar
  6. 6.
    Sikkema, J., de Bont, J.A.M., and Poolman, B., Microbiol. Rev., 1995, vol. 59, no. 2, pp. 201–222.PubMedPubMedCentralGoogle Scholar
  7. 7.
    de Carvahlo, C.C.C.R., in Biology of Rhodococcus. Microbiology Monographs 16, Alvares, H.M., Ed., Berlin: Springer-Verlag, 2010, pp. 109–131.Google Scholar
  8. 8.
    Sardessai, Y.N. and Bhosle, S., Biotechnol. Prog., 2004, vol. 20, no. 3, pp. 655–660.CrossRefPubMedGoogle Scholar
  9. 9.
    Isken, S. and de Bont, J.A.M., Extremophiles, 1998, vol. 2, no. 3, pp. 229–238.CrossRefPubMedGoogle Scholar
  10. 10.
    Newmann, G., Veeranagouda, Y., Karegoudar, T.B., Sahim, O., Mausezahl, I., Kabelitz, N., Kappelmeyer, U., and Heipieper, H.J., Extremophiles, 2005, vol. 9, no. 2, pp. 163–168.CrossRefGoogle Scholar
  11. 11.
    Kongpol, A., Kato, J., Tajima, T., and Vangnai, A.S., Microbes Environ., 2012, vol. 27, no. 1, pp. 30–35.CrossRefPubMedGoogle Scholar
  12. 12.
    Valyshev, A.V. and Vasil’chenko, A.S., Zh. Mikrobiol. Epidemiol. Immunol., 2013, no. 5, pp. 8–13.Google Scholar
  13. 13.
    Fantner, G.E., Barbero, R.J., Gray, D.S., and Belcher, A.M., Nat. Nanotechnol., 2010, vol. 5, no. 4, pp. 280–285.CrossRefPubMedPubMedCentralGoogle Scholar
  14. 14.
    Gaur, R. and Khare, S.K., Lett. Appl. Microbiol., 2009, vol. 49, no. 3, pp. 372–377.CrossRefPubMedGoogle Scholar
  15. 15.
    Yamashita, S., Satoi, M., Iwasa, Y., Honda, K., Sameshima, Y., Omasa, T., Kato, J., and Ohtake, H., Appl. Microbiol. Biotechnol., 2007, vol. 74, no. 4, pp. 761–767.CrossRefPubMedGoogle Scholar
  16. 16.
    El’kin, A.A., Grishko, V.V., and Ivshina, I.B., Prikl. Biokhim. Mikrobiol., 2010, vol. 46, no. 6, pp. 637–643.PubMedGoogle Scholar
  17. 17.
    Nogovitsina, E.M., Grishko, V.V., and Ivshina, I.B., Bioorg. Khim., 2011, vol. 37, no. 5, pp. 697–704.PubMedGoogle Scholar
  18. 18.
    Grishko, V.V., Tarasova, E.V., and Ivshina, I.B., Proc. Biochem. Soc., 2013, vol. 4, no. 11, p. 1640.CrossRefGoogle Scholar
  19. 19.
    Ivshina, I.B., Kuyukina, M.S., Plilp, J.C., and Christofi, N., J. Microbiol. Biotechnol., 1998, vol. 14, no. 5, pp. 711–717.CrossRefGoogle Scholar
  20. 20.
    Kuyukina, M.S., Ivshina, I.B., Korshunova, I.O., and Rubtsova, E.V., J. Microbiol. Methods, 2014, vol. 107, pp. 23–29.CrossRefPubMedGoogle Scholar
  21. 21.
    Keits, M., Techniques of Lipidology: Isolation, Analysis, and Identification of Lipids, Amsterdam: Elsevier, 1972.Google Scholar
  22. 22.
    Ivshina, I.B., Kamenskikh, T.N., and Anokhin, B.A., Vestnik Permskogo Universiteta, 2007, vol. 10, no. 5, pp. 107–112.Google Scholar
  23. 23.
    de Carvalho, C.C.C.R., Poretti, A., and Fonseca, M.M.R., Appl. Microbiol. Biotechnol., 2005, vol. 69, no. 3, pp. 268–275.CrossRefPubMedGoogle Scholar
  24. 24.
    de Carvalho, C.C.C.R., Cruz, A.A.R.L., Pons, M.-N., Pinheiro, H.M.R.V., Cabral, J.M.S., da Fonseca, M.M.R., Ferreira, B.S., and Fernandes, P., Microsc. Res. Tech., 2004, vol. 64, no. 3, pp. 215–222.CrossRefPubMedGoogle Scholar
  25. 25.
    Dufrene, Y.F., J. Bacteriol., 2002, vol. 184, no. 19, pp. 5205–5213.CrossRefPubMedPubMedCentralGoogle Scholar
  26. 26.
    Dorobantu, L.S., Goss, G.G., and Burrell, R.E., Micron, 2012, vol. 43, no. 12, pp. 1312–1322.CrossRefPubMedGoogle Scholar
  27. 27.
    Rubtsova, E.V., Kuyukina, M.S., and Ivshina, I.B., Appl. Biochem. Microbiol., 2012, vol. 48, no. 5, pp. 452–459.CrossRefGoogle Scholar
  28. 28.
    Kuyukina, M.S., Ivshina, I.B., Rychkova, M.I., and Chumakov, O.B., Mikrobiologiya, 2000, vol. 69, no. 1, pp. 62–69.Google Scholar
  29. 29.
    Ramos, J.L., Duque, E., Gallegos, M.-T., Godoy, P., Ramos-Gonzales, M.I., Rojas, A., Teran, W., and Segura, A., Annu. Rev. Microbiol., 2002, vol. 56, pp. 743–768.CrossRefPubMedGoogle Scholar
  30. 30.
    Kieboom, J., Dennis, J.J., Zylstra, G.J., and de Bont, J.A.M., J. Bacteriol., 1998, vol. 180, no. 24, pp. 6769–6772.PubMedPubMedCentralGoogle Scholar
  31. 31.
    Gressler, L.T., de Vargas, A.C., Costa, M.M., and Potter, L., Da silveira bd., sangioni la., de avila botton s, Braz. J. Microbiol., 2014, vol. 45, no. 2, pp. 661–665.Google Scholar
  32. 32.
    de Rossi, E., Ainsa, J.A., and Riccardi, G., FEMS Microbiol. Rev., 2006, vol. 30, no. 1, pp. 36–52.CrossRefPubMedGoogle Scholar
  33. 33.
    Blair, J.M.A. and Piddock, L.J.V., Curr. Opin. Microbiol., 2009, vol. 12, no. 5, pp. 512–519.CrossRefPubMedGoogle Scholar

Copyright information

© Pleiades Publishing, Inc. 2016

Authors and Affiliations

  • I. O. Korshunova
    • 1
  • O. N. Pistsova
    • 2
  • M. S. Kuyukina
    • 1
    • 2
  • I. B. Ivshina
    • 1
    • 2
  1. 1.Institute of Ecology and Genetics of MicroorganismsRussian Academy of SciencesPermRussia
  2. 2.Perm State UniversityPermRussia

Personalised recommendations