Applied Biochemistry and Microbiology

, Volume 51, Issue 1, pp 58–63 | Cite as

Amylase inhibitors from Streptomyces lucensis VKPM Ac-1743 and Streptomyces violaceus VKPM Ac-1734



Inhibitors synthesized by the Streptomyces lucensis VKPM AS-1743 and Streptomyces violaceus VKPM AS-1734 strains were studied for their influence on amylases of different origin. The effect of the inhibitors was shown to be different on fungal amylase, pancreatic amylase, and amylase from human blood. It has been found that the studied inhibitors are substances of a pseudooligosaccharide nature and exhibit their activity and stability over a wide range of pH and temperature values. The physico-chemical and biochemical properties of isolated inhibitors were compared with those of known microbial inhibitors of α-glucosidases.


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  1. 1.
    Akulova, N.Yu. and Selezneva, A.A., Prikl. Biokhim. Mikrobiol., 1995, vol. 31, no. 4, pp. 371–380.Google Scholar
  2. 2.
    Wehmeier, U.F., Acarbose-ein therapeutisch eingesetzter wirkstoff: biosynthese und funktion, Biospektrum, 2004, no. 1, pp. 34–36.Google Scholar
  3. 3.
    Yi, Yu., Appl. Environ. Microbiol., 2005, vol. 71, no. 9, pp. 5066–5076.CrossRefGoogle Scholar
  4. 4.
    Yamagishi, T., Uchida, C., and Ogawa, S., Chemistry-Eur. J., 2006, vol. 1, no. 9, pp. 634–636.CrossRefGoogle Scholar
  5. 5.
    Withers, R.A., Glycobiology, 2008, vol. 18, pp. 570–586.PubMedCrossRefGoogle Scholar
  6. 6.
    Chen, X., Zheng, Y., and Shen, Y., Curr. Med. Chem., 2006, vol. 13, no. 1, pp. 109–116.PubMedCrossRefGoogle Scholar
  7. 7.
    Wang, H., Ni, Y., Yang, S., Li, H., Li, X., and Feng, B., Curr. Ther. Res., 2013, vol. 75, pp. 88–92.PubMedCentralPubMedCrossRefGoogle Scholar
  8. 8.
    Scott, L.J. and Spencer, C.M., Drugs, 2000, vol. 59, pp. 521–549.PubMedCrossRefGoogle Scholar
  9. 9.
    US Patent No. 7056900, 2006.Google Scholar
  10. 10.
    Stephens, J.M., Small Peptide Analogues of the α-Amylase Inhibitoring Ptotein, Ttndamistat, 2005.Google Scholar
  11. 11.
    RF Patent No. 2355755, 2009.Google Scholar
  12. 12.
    RF Patent No. 2346042, 2009.Google Scholar
  13. 13.
    RF Patent No. 2495930, 2013.Google Scholar
  14. 14.
    Zakharova, I.Ya. and Kosenko, L.V., Metody izucheniya mikrobnykh polisakharidov (Methods for Studying Microbial Polysaccharides), Kiev: Naukova dumka, 1982.Google Scholar
  15. 15.
    Xiao, Z., Storms, R., and Tsang, A., Anal. Biochem., 2006, vol. 351, no. 1, pp. 146–148.PubMedCrossRefGoogle Scholar
  16. 16.
    Yoshino, M. and Murakami, K.J., Enzyme Inhib. Med. Chem., 2009, vol. 24, no. 6, pp. 1288–90.CrossRefGoogle Scholar
  17. 17.
    Patent RF No. 748444, 1996.Google Scholar
  18. 18.
    Bezborodov, A.M., Mikrobnye metabolity ingibitory fermentov (Microbial Metabolites-Enzyme Inhibitors), Moscow: Nauka, 1986.Google Scholar
  19. 19.
    Krasikov, V.V., Karelov, D.V., and Firsov, L.M., Biokhimiya, 2001, vol. 66, no. 3, pp. 332–348.Google Scholar
  20. 20.
    Robyt, J.F., Biologia, 2005, vol. 60, no. 16, pp. 17–26.Google Scholar
  21. 21.
    Sigurskjold, B.W., Christensen, U., Frandsen, T.P., Mirgorodskaya, E., Harrison, M., Roepstor, P., and Svensson, B., Biochem. Biophys. Acta, 2000, vol. 1543, pp. 275–293.PubMedGoogle Scholar
  22. 22.
    Sharova, N.Yu. and Selezneva, A.A., Appl. Biochem. Microbiol., 1999, vol. 35, no. 1, pp. 8–1.Google Scholar
  23. 23.
    Wang, H., Ni, Y., Yang, S., Li, H., Li, X., and Feng, B., Curr. Ther. Res. Clin. Exp., 2013, vol. 75, pp. 88–92.PubMedCentralPubMedCrossRefGoogle Scholar
  24. 24.
    Lee, M.Y., Choi, D.S., Lee, M.K., Lee, H.W., Park, T.S., Kim, D.M., Chung, C.H., Kim, D.K., Kim, I.J., Jang, H.C., Park, Y.S., Kwon, H.S., Lee, S.H., and Shin, H.K., J. Korean Med Sci., 2014, vol. 29, no. 1, pp. 90–97.PubMedCentralPubMedCrossRefGoogle Scholar
  25. 25.
    Xue, Y.-P., Zheng, Y.-G., Chen, X.-L., and Shen, Y.-C., J. Chromatogr. Sci., 2007, vol. 45, pp. 87–90.PubMedCrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Inc. 2015

Authors and Affiliations

  1. 1.All-Russia Research Institute of Food Flavorings, Acids and Dyes AdditivesSt. PetersburgRussia

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