Applied Biochemistry and Microbiology

, Volume 50, Issue 7, pp 730–736 | Cite as

Preparation and characterization of bioactive products obtained via the solubilization of brown coal by white rot fungi

  • O. I. Klein
  • N. A. Kulikova
  • E. V. Stepanova
  • O. I. Filippova
  • T. V. Fedorova
  • L. G. Maloshenok
  • I. S. Filimonov
  • O. V. Koroleva
Application of Biomaterials

Abstract

We investigated the solubilizing activity of the Basidiomycete fungi Trametes hirsuta and Trametes maxima, with respect to brown coal (lignite) during liquid phase cultivation. We found that the degrading capacity of the fungi is determined by the activity of the ligninolytic enzymes Mn peroxidase and lignin peroxidase. We assessed the growth-stimulating activity of biopreparations (BPs), based on the culture liquids (CL) of the studied fungal strains, which were grown on a rich or minimal medium. We found that the obtained BPs inhibited the growth of wheat shoots and roots at the germination stage, but they either had no effect at later stages of plant growth or showed a mild stimulation. When basidiomycetes were cultivated in the presence of brown coal, the obtained BPs stimulated root growth at the germination stage, and did not influence plant growth (Trametes hirsuta) or stimulated it (Trametes maxima) at later stages. Further, we report a pronounced detoxifying ability of the BPs in respect to the atrazine herbicide. We suggest that this effect is caused by the laccases action, that are present in the studied BPs.

Key words

biosolubilization brown coal lingninolytic enzymes Trametes hirsuta Trametes maxima 

Abbreviations

BP

biopreparations

HS

humic substances

CL

culture liquid

IU

international unit of enzyme activity

PSII

photosynthesis system II

ETC

electron transport chain

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Copyright information

© Pleiades Publishing, Inc. 2014

Authors and Affiliations

  • O. I. Klein
    • 1
  • N. A. Kulikova
    • 1
    • 2
  • E. V. Stepanova
    • 1
  • O. I. Filippova
    • 2
  • T. V. Fedorova
    • 1
  • L. G. Maloshenok
    • 1
  • I. S. Filimonov
    • 3
  • O. V. Koroleva
    • 1
  1. 1.The Bach Institute of BiochemistryRussian Academy of Sciences (RAS)MoscowRussia
  2. 2.The Department of Soil ScienceLomonosov Moscow State UniversityMoscowRussia
  3. 3.The International Education Research Center of BiotechnologyLomonosov Moscow State UniversityMoscowRussia

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