Applied Biochemistry and Microbiology

, Volume 50, Issue 2, pp 125–133 | Cite as

Microbial degradation of chitin waste for production of chitosanase and food related bioactive compounds

  • S. SinhaEmail author
  • S. Chand
  • P. Tripathi


Ecological samples rich in microbial diversity like cow dung, legume rhizosphere, fish waste and garden soil were used for isolation of chitosan-degrading microorganisms. Selected isolates were used for production of chitosanaseand food related bioactive compounds by conversion of biowaste. Production of glucosamine (Gln), N-acetylglucosamine (NAG), chitooligosaccharides (COS), antioxidants, antibacterial compounds and prebiotics was carried out by microbial fermentation of biowaste. The highest chitosanase activity (8 U/mL) was observed in Aspergillus sp. isolated from fish market waste and it could produce Gln and NAG while Streptomyces sp. isolated from garden soil was able to produce COS along with Gln and NAG. Radical scavenging activity was observed in culture supernatants of 35% of studied isolates, and 20% isolates secreted compounds which showed positive effect on growth of Bifidobacterium. Antibacterial compounds were produced by 40% of selected isolates and culture supernatants of two microbial isolates, Streptomyces zaomyceticus C6 and one of garden soil isolates, were effective against both gram positive and negative bacteria.


Chitosan Chitin DPPH Apply Biochemistry Biowaste 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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© Pleiades Publishing, Inc. 2014

Authors and Affiliations

  1. 1.Department of Biochemical Engineering and BiotechnologyIndian Institute of TechnologyNew Delhi-16India
  2. 2.Schools of SciencesIndira Gandhi National Open UniversityNew Delhi-68India

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