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Applied Biochemistry and Microbiology

, Volume 49, Issue 6, pp 581–586 | Cite as

Production of extracellular proteinases—protein C activators of blood plasma—by the micromycete Aspergillus ochraceus during submerged and solid-state fermentation

  • A. A. OsmolovskyEmail author
  • V. G. Kreier
  • N. A. Baranova
  • A. V. Kurakov
  • N. S. Egorov
Article

Abstract

The conditions for the submerged and solid-state fermentation of the micromycete Aspergillus ochraceus VKM F-4104D, producing extracellular proteinases that activate protein C of human blood plasma, were optimized. It is shown that the protein C-activating activity of the micromycete in a solid-state culture was 1.5-3.5 times higher than in a submerged culture (as calculated per milliliter of culture medium). Among the extracellular proteins secreted by A. ochraceus VKM F-4104D during submerged and solid-state fermentation, a protein C-activating proteinase with a pI of 6.0–6.3 was identified.

Keywords

Fermentation Apply Biochemistry Wheat Bran Solid State Fermentation Activate Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Pleiades Publishing, Inc. 2013

Authors and Affiliations

  • A. A. Osmolovsky
    • 1
    Email author
  • V. G. Kreier
    • 1
  • N. A. Baranova
    • 1
  • A. V. Kurakov
    • 1
  • N. S. Egorov
    • 2
  1. 1.Biological FacultyMoscow State UniversityMoscowRussia
  2. 2.International Biotechnological CenterMoscow State UniversityMoscowRussia

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