Applied Biochemistry and Microbiology

, Volume 49, Issue 3, pp 306–311 | Cite as

Detection of pyrethroids by spectral correlation interferometry

  • A. G. BureninEmail author
  • M. P. Nikitin
  • A. V. Orlov
  • T. I. Ksenevich
  • P. I. Nikitin


A label-free method based on spectral correlation interferometry has been developed for highly sensitive detection of pyrethroids by competitive immunoassay on the surface of sensor chips made of widely available microscopy glass cover slips. It is shown that the method allows independent optimization of each step of the sensor surface modification. This fact may be used to increase the efficiency of development of protocols for a wide spectrum of immunoassays that employ glass surface as a solid phase. Detection of 3-phenoxybenzoic acid, which is one of the most stable metabolites of a large number of pyrethroids, on the surface of the optimized sensor chips has been demonstrated on the level of 15 pg/ml. That is 50 times better than the sensitivity of the enzyme-linked immunosorbent assay (ELISA).


Apply Biochemistry Surface Plasmon Resonance Delta Methrin Sensor Chip DMFA 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



surface plasmon resonance




3-phenoxybenzoic acid


phosphate buffered saline






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Copyright information

© Pleiades Publishing, Ltd. 2013

Authors and Affiliations

  • A. G. Burenin
    • 1
    Email author
  • M. P. Nikitin
    • 1
  • A. V. Orlov
    • 1
  • T. I. Ksenevich
    • 2
  • P. I. Nikitin
    • 2
  1. 1.Moscow Institute of Physics and TechnologyMoscowRussia
  2. 2.Prokhorov General Physics InstituteMoscowRussia

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