Applied Biochemistry and Microbiology

, Volume 48, Issue 5, pp 519–524 | Cite as

Receptor elements for biosensors in two ways of methylotrophic yeast immobilization

  • M. G. Zaitsev
  • V. A. Arlyapov
  • V. A. Alferov
  • A. N. Reshetilov


Receptor elements for biosensors based on Hansenula polymorpha NCYC 495 ln yeast cells for ethanol assay were developed using two ways of cell immobilization, i.e., physical adsorption on a glass fiber membrane and covalent binding on a modified nitrocellulose membrane. The linear diapason of ethanol assays for a biosensor based on yeast cells adsorbed on glass fiber was 0.05–1.18; for a biosensor based on yeasts immobilized on a nitrocellulose membrane, 0.2–1.53 mM. Receptor elements based on sorbed cells possessed 2.5 times higher long-term stability. The time response was 1.5 times less for cells immobilized using DEAE-dextran and benzoquinone. The results of ethyl alcohol assays using biosensors based on cells immobilized via adsorption and covalent binding, as well as using the standard areometric method, had high correlation coefficients (0.998 and 0.997, respectively, for the two ways of immobilization). The results indicate the possibility to consider the described models of receptor elements for biosensors as prototypes for experimental samples for practical use.


Immobilization Yeast Cell Apply Biochemistry Covalent Binding Methylotrophic Yeast 
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Copyright information

© Pleiades Publishing, Ltd. 2012

Authors and Affiliations

  • M. G. Zaitsev
    • 1
  • V. A. Arlyapov
    • 1
  • V. A. Alferov
    • 1
  • A. N. Reshetilov
    • 2
  1. 1.Tula State UniversityTulaRussia
  2. 2.Institute of the Biochemistry and Physiology of MicroorganismsRussian Academy of SciencesPushchino, Moscow oblastRussia

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