Applied Biochemistry and Microbiology

, Volume 47, Issue 3, pp 298–303 | Cite as

Melanin of Laetiporus sulphureus (Bull.: Fr.) Murr sterile form

  • D. N. OlennikovEmail author
  • S. V. Agafonova
  • A. V. Stolbikova
  • A. V. Rokhin


Melanin complex was isolated from mycelium of the basidiomycete Laetiporus sulphureus (Bull.: Fr.) Murr (with a yield of 2.49% of the dry weight). UV and IR spectroscopies, gel chromatography, and alkaline cleavage assay demonstrated that the isolated melanin was heterogeneous and belonged to the dihydronaphthalene type. 13C-NMR data suggested that aromatic fragments were dominant in the melanin structure. In vitro study of the antioxidant action demonstrated that the L. sulphureus melanin displayed a radical-scavenging activity and the ability to inactivate hydrogen peroxide and nitrogen(II) oxide molecules and to chelate iron(II) ions.


DPPH Apply Biochemistry Alter Naria Alternata High Performance Thin Layer Chromatography Alkaline Cleavage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Bell, A.A. and Wheeler, M.H., Annu. Rev. Phytopathol., 1986, vol. 24, no. 3, pp. 411–451.CrossRefGoogle Scholar
  2. 2.
    Jacobson, E.S., Clin. Microbiol. Rev., 2000, vol. 13, no. 4, pp. 708–717.PubMedCrossRefGoogle Scholar
  3. 3.
    Borshchevskaya, M.I. and Vasil’eva, S.M., Vopr. Med. Khim., 1999, vol. 45, no. 1, pp. 13–23.Google Scholar
  4. 4.
    Zherebin, Yu.A., Dokl. AN USSR. Seriya B, 1984, no. 3, pp. 64–68.Google Scholar
  5. 5.
    Grigoryan, E.N. and Mitashov, V.I., Ontogenez, 1979, vol. 10, no. 2, pp. 137–144.Google Scholar
  6. 6.
    Babitskaya, V.G., Shcherba, V.V., and Ikonnikova, N.V., Prikl. Biokhim. Mikrobiol., 2000, vol. 36, no. 4, pp. 439–444.Google Scholar
  7. 7.
    Shashkina, M.Ya., Shashkin, P.N., and Sergeev, A.V., Pharm. Chem. J., 2006, vol. 40, no. 10, pp. 560–568.CrossRefGoogle Scholar
  8. 8.
    Agafonova, S.V., Olennikov, D.N., Borovskii, G.B., and Penzina, T.A., Chem. Natural Comp., 2007, vol. 43, no. 6, pp. 687–688.CrossRefGoogle Scholar
  9. 9.
    Olennikov, D.N., Agafonova, S.V., Borovskii, G.B., Penzina, T.A., and Rokhin, A.V., Prikl. Biokhim. Mikrobiol., 2009, vol. 45, no. 5, pp. 597–605.PubMedGoogle Scholar
  10. 10.
    Olennikov, D.N., Agafonova, S.V., Borovskii, G.B., Penzina, T.A., and Rokhin, A.V., Prikl. Biokhim. Mikrobiol., 2009, vol. 45, no. 6, pp. 693–697.PubMedGoogle Scholar
  11. 11.
    Agafonova, S.V., Borovskii, G.B., Penzina, T.A., and Olennikov, D.N., Usp. Med. Mikol., 2007, vol. 9, pp. 208–209.Google Scholar
  12. 12.
    Olennikov, D.N., Agafonova, S.V., Nazarova, A.V., Borovskii, G.B., and Penzina, T.A., Chem. Natural Comp., 2008, vol. 44, no. 6, pp. 762–763.CrossRefGoogle Scholar
  13. 13.
    Koroleva, O.V., Kulikova, N.A., Alekseeva, T.N., Stepanova, E.V., Davidchuk, V.N., Belyaeva, E.Yu., and Tsvetkova, E.A., Prikl. Biokhim. Mikrobiol., 2007, vol. 43, no. 1, pp. 61–67.Google Scholar
  14. 14.
    Seyoum, A., Asres, K., and El-Fiky, F.K., Phytochemistry, 2006, vol. 67, no. 18, pp. 2058–2070.PubMedCrossRefGoogle Scholar
  15. 15.
    Chen, A.-S., Taguchi, T., Sakai, K., Kikuchi, K., Wang, M.-W., and Miwa, I., Biol. Pharm. Bull., 2003, vol. 26, no. 9, pp. 1326–1330.PubMedCrossRefGoogle Scholar
  16. 16.
    Govindarajan, R., Rastogi, S., and Vijayakumar, M., Biol. Pharm. Bull., 2003, vol. 26, no. 10, pp. 1424–1427.PubMedCrossRefGoogle Scholar
  17. 17.
    Olennikov, D.N., Zilfikarov, I.N., Toropova, A.A., and Ibragimov, T.A., Khim. Rastit. Syr’ya, 2008, no. 4, pp. 95–100.Google Scholar
  18. 18.
    Rukovodstvo po eksperimental’nomu (doklinicheskomu) izucheniyu novykh farmakologicheskikh veshchestv (Guide to Experimental (Preclinical) Study of New Pharmacological Agents), Khabriev, R.U., Ed., Moscow: Meditsina, 2000, pp. 18–24.Google Scholar
  19. 19.
    Metner, J.M., Townsel, M.E., Moore, C.L., Williamson, G.D., Soteres, B.J., Fisher, M.S., and Willis, I., Pigm. Cell Res., 1990, vol. 3, no. 2, pp. 90–97.CrossRefGoogle Scholar
  20. 20.
    Modern Methods of Plant Analysis, Paech, K. and Tracey, M.V., Eds., Berlin: Springer, 1955, pp. 661–675.Google Scholar
  21. 21.
    Suryanarayanan, T.S., Ravishankar, J.P., Venkatesan, G., and Murali, Th.S., Mycol. Res., 2004, vol. 108, no. 8, pp. 974–978.PubMedCrossRefGoogle Scholar
  22. 22.
    Pierce, J.H. and Rast, D.M., Phytochemistry, 1995, vol. 39, no. 1, pp. 49–55.CrossRefGoogle Scholar
  23. 23.
    Knicker, H., Almendros, G., Gonzalez-Vila, F.J., Lodemann, H.-D., and Martin, F., Org. Geochem., 1995, vol. 23, nos. 11–12, pp. 1023–1028.CrossRefGoogle Scholar
  24. 24.
    Babitskaya, V.G., Shcherba, V.V., Filimonova, T.V., and Grigorchuk, E.A., Prikl. Biokhim. Mikrobiol., 2000, vol. 36, no. 2, pp. 153–159.Google Scholar
  25. 25.
    Paim, S., Linhares, L.F., Mangrich, A.S., and Martin, J.P., Biol. Fertil. Soils, 1990, vol. 10, no. 1, pp. 72–76.Google Scholar
  26. 26.
    De Angelis, F., Arcadi, A., Marinelli, F., Paci, M., Botti, D., Pacioni, G., and Miranda, M., Phytochemistry, 1996, vol. 43, no. 5, pp. 1103–1106.CrossRefGoogle Scholar
  27. 27.
    Butler, M.J., Gardiner, R.B., and Day, A.W., Mycologia, 2009, vol. 101, no. 3, pp. 296–304.PubMedCrossRefGoogle Scholar
  28. 28.
    Babitskaya, V.G. and Shcherba, V.V., Prikl. Biokhim. Mikrobiol., 2002, vol. 38, no. 3, pp. 286–291.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  • D. N. Olennikov
    • 1
    Email author
  • S. V. Agafonova
    • 2
  • A. V. Stolbikova
    • 2
  • A. V. Rokhin
    • 3
  1. 1.Institute of General and Experimental Biology, Siberian BranchRussian Academy of SciencesUlan-UdeRussia
  2. 2.Siberian Institute of Plant Physiology and Biochemistry, Siberian BranchRussian Academy of SciencesIrkutskRussia
  3. 3.Irkutsk State UniversityIrkutskRussia

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