Applied Biochemistry and Microbiology

, Volume 46, Issue 8, pp 755–762 | Cite as

Larvicidal activity of crystal-forming strains of Brevibacillus laterosporus

  • M. V. Zubasheva
  • L. A. Ganushkina
  • T. A. Smirnova
  • R. R. Azizbekyan
Article

Abstract

The optimum conditions for growth, sporulation, and crystal-formation in four isolated crystal-forming strains of Bacillus laterosporus were determined. It was shown that culture broth and pellets of bacterial culture liquid possess larvicidal activity against larvae of mosquitoes A. stephensi and A. aegypti. The protein nature of crystal was shown. Crystals are monocomponent containing a protein with MM of 68 or 130 kDa. Purified protein crystals demonstrated larvicidal activity. Specific larvicidal activity of crystals of various strains essentially differed. High larvicidal activity of B. laterosporus strains allows for them to be recommended as producers of antimosquito biological preparations.

Key words

Brevibacillus laterosporus entomocidal crystal larvicidal activity mosquito 

Abbreviations

DTT

dithiotreitol

DFP

diisopropylfluorophosphate

CL

cultural liquid

CFU

colony-forming unit

OD

optical density

PAAG

polyacrylamidegel

PMSF

phenylmethylsolfonylfluoride

EDTA

ethylendiaminetetraacetic acid

SDS

sodium dodecylsulphat

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Shida, O., Takagi, H., Kadowaki, K., and Komagata, K., Int. J. Syst. Bacteriol., 1996, vol. 46, pp. 939–946.CrossRefPubMedGoogle Scholar
  2. 2.
    Orlova, M.V., Smirnova, T.A., Shamshina, T.N., Kovalenko, N.A., and Azizbekyan, R.R., Biotekhnologiya, 1995, nos. 1–2, pp. 22–26.Google Scholar
  3. 3.
    Gerard, J.M., Haden, P., Kellyk, M.T., and Andersen, R.J., J. Nat. Prod., 1999, vol. 62, no. 1, pp. 80–85.CrossRefPubMedGoogle Scholar
  4. 4.
    Desjardine, K., Pereira, A., Wright, H., Matainaho, T., Kelly, M., and Andersen, R.J., J. Nat. Prod., 2007, vol. 70, no. 12, pp. 1850–1853.CrossRefPubMedGoogle Scholar
  5. 5.
    Barsby, T., Kelly, M.T., and Andersen, R.J., N. Nat. Prod., 2002, vol. 65, no. 10, pp. 1447–1451.CrossRefGoogle Scholar
  6. 6.
    Favret, M.E. and Yousten, A.A., J. Invertebr. Pathol., 1985, vol. 45, pp. 195–203.CrossRefPubMedGoogle Scholar
  7. 7.
    Orlova, M.V., Smirnova, T.A., Ganushkina, L.A., and Azizbekyan, R.R., Appl. Environ. Microbiol., 1998, vol. 64, pp. 2723–2725.PubMedGoogle Scholar
  8. 8.
    Ruiu, L., Delrio, G., Ellar, D., Floris, I., Paglietti, B., Rubino, S., and Satta, A., Entomol. Exper. Appl., 2006, vol. 118, pp. 137–144.CrossRefGoogle Scholar
  9. 9.
    de Oliveira, E.J., Rabinovitch, L., Monnerat, R.G., Konovaloff, L., Jannotti, P.J., and Zahner, V., Appl. Enriron. Microbiol., 2004, vol. 70, no. 11, pp. 6657–6664.CrossRefGoogle Scholar
  10. 10.
    Huang, X., Tian, B., Niu, Q., Yang, J., Zhang, L., and Zhang, K., Res. Microbiol., 2005, vol. 156, nos. 5–6, pp. 719–727.CrossRefPubMedGoogle Scholar
  11. 11.
    Donnell, B., US Patent no. 5702701, C12N001/29, 1997.Google Scholar
  12. 12.
    Sanders, M.E., Morelli, L., and Tompkins, T.A., Comp. Rev. Food Sci. Food Saf., 2006, vol. 2, pp. 101–110.CrossRefGoogle Scholar
  13. 13.
    Kamiyama, T., Umino, T., Nakamura, Y., Itezono, Y., Sawairi, S., Saton, T., and Yokose, K., J. Antibiot., 1994, vol. 47, pp. 959–968.PubMedGoogle Scholar
  14. 14.
    Bachtel, D.B. and Bulla, L.A., J. Bacteriol., 1979, vol. 127, pp. 1472–1481.Google Scholar
  15. 15.
    Charles, J.F., Nielsen-Leroux, C., Delecluse, A., and Charles, J.F., Ann. Rev. Entomol., 1996, vol. 41, pp. 451–472.CrossRefGoogle Scholar
  16. 16.
    Rivers, D.B., Vann, C.N., Zimmack, H.L., and Dean, D.H., J. Invertbr. Pathol., 1991, vol. 58, pp. 444–447.CrossRefGoogle Scholar
  17. 17.
    Smirnova, T.A., Shamshina, T.N., Konstantinova, G.E., Gannushkina, L.A., Kuznetsova, N.I., Nikolaenko, M.A., and Azizbekyan, R.R., Biotekhnologiya, 1993, no. 9, pp. 11–15.Google Scholar
  18. 18.
    Zeigler, D.R., Bacillus Genetic Stock Center. Catalog of Strains, 7th ed., Ohio: The Ohio State University, 1999, vol. 2, pp. 1–56.Google Scholar
  19. 19.
    Thorne, C., J. Virol., 1968, vol. 2, pp. 657–661.PubMedGoogle Scholar
  20. 20.
    Nickerson, K.W. and Bulla, L.A., Appl. Microbiol., 1974, vol. 28, pp. 124–128.PubMedGoogle Scholar
  21. 21.
    Nakata, H.M. and Halvorson, H.O., J. Bacteriol., 1960, vol. 80, pp. 801–810.PubMedGoogle Scholar
  22. 22.
    Zalunin, I.A., Kostina, L.I., Chestukhina, G.G., Brmatova, M.E., Klepikova, F.S., Khodova, O.M., and Stepanov, V.M., Biokhimiya, 1986, vol. 51, no. 3, pp. 449–457.Google Scholar
  23. 23.
    Pfannenstiel, M.A., Crey, W.C., Jr., Couche, G., and Nickerson, K.W., Appl. Environ. Microbiol., 1990, vol. 56, no. 1, pp. 162–166.PubMedGoogle Scholar
  24. 24.
    Ibarra, J.E. and Fererici, B.A., J. Bacteriol., 1986, vol. 165, no. 2, pp. 527–533.PubMedGoogle Scholar
  25. 25.
    Smirnoff, W.A., J. Insect. Pathol., 1962, vol. 4, pp. 384–386.Google Scholar
  26. 26.
    Chestukhina, G.G., Zalunin, I.A., Kostina, L.I., Kotova, T.S., Katrukha, S.P., Lyublinskaya, L.A., and Stepanov, V.M., Biokhimiya, 1978, vol. 43, no. 5, pp. 857–864.Google Scholar
  27. 27.
    Bradford, M., Anal. Biochem., 1976, vol. 72, pp. 248–254.CrossRefPubMedGoogle Scholar
  28. 28.
    Zalunin, I.A., Chestukhina, G.G., and Stepanov, V.M., Biokhimiya, 1979, vol. 44, no. 4, pp. 693–698.Google Scholar
  29. 29.
    Laemmli, U.K., Nature (London), 1970, vol. 227, pp. 680–685.CrossRefGoogle Scholar
  30. 30.
    Voitsik, A.A. and Rasnitsyn, S.P., Med. Parazitol., 1992, no. 4, pp. 55–57.Google Scholar
  31. 31.
    Claus, D. and Berkeley, R.C.W., in Bergey’s Manual of Systematic Bacteriology, Sneath, P.H.A., Mair, N.S., Sharpe, M.E., and Holt, J.G., Eds., Baltimore: Williams Wilkins, 1986, vol. 2, pp. 1105–1139.Google Scholar
  32. 32.
    Charles, J.F. and de Barjac, H., An. Microbiol. (Inst. Pasteur), 1982, vol. 133A, pp. 425–442.Google Scholar
  33. 33.
    Smirnova, T.A., Minenkova, I.B., Orlova, M.V., Lecadet, M.-M., and Azizbekyan, R.R., Res. Microbiol., 1996, vol. 47, pp. 343–350.CrossRefGoogle Scholar
  34. 34.
    Smirnova, T.A., Poglazova, M.N., Orlova, M.V., and Azizbekyan, R.R., Biotekhnologiya, 1997, no. 1, pp. 29–36.Google Scholar
  35. 35.
    Smirnova, T.A. and Azizbekyan, R.R., Biotekhnologiya, 2002, no. 5, pp. 8–13.Google Scholar
  36. 36.
    Fitz-James, P.C. and Young, I.E., J. Biochem. Cytol., 1958, vol. 4, pp. 639–649.CrossRefGoogle Scholar
  37. 37.
    Lecadet, M.-M., Microbial. Toxins, 1970, vol. 3, pp. 437–440.Google Scholar
  38. 38.
    Insell, J.P. and Fitz-James, P.C., Appl. Environ. Microbiol., 1985, vol. 50, pp. 56–62.PubMedGoogle Scholar
  39. 39.
    Grigor’eva, T.M. and Azizbekyan, R.R., Biotekhnologiya, 2002, no. 4, pp. 31–37.Google Scholar
  40. 40.
    Charles, J.F. and Nielsen-Leroux, C., Mem. Inst. Oswaldo Cruz, 2000, vol. 95, pp. 201–206.CrossRefPubMedGoogle Scholar
  41. 41.
    Grigor’eva, T.M. and Azizbekyan, R.R., in Mat. 2-go Mezhdunarodnogo kongressa “Biotekhnologiya-sostoyanie i perspektivy razvitiya” (Proc. 2nd Int. Congr. “Biotechnology: State and Prospects of Development”), Moscow, 2003.Google Scholar
  42. 42.
    Chestukhina, G.G., Kotova, T.S., Zalunin, I.A., and Stepanov, V.M., Biokhimiya, 1979, vol. 44, pp. 796–802.Google Scholar
  43. 43.
    Federici, B.A., Luthy, P., and Ibarra, J.A., in Bacterial Control of Mosquitoes and Black Flies: Biochemistry, Genetics and Application of Bacillus thuringiensis israelensis and Bacillus sphaericus, de Barjak, H. and Sazerland, D.J., Eds., Rutgers: University Press, 1990, pp. 16–44.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  • M. V. Zubasheva
    • 1
  • L. A. Ganushkina
    • 2
  • T. A. Smirnova
    • 1
  • R. R. Azizbekyan
    • 1
  1. 1.State Research Institute for Genetics and Selection of Industrial MicroorganismsMoscowRussia
  2. 2.Martsinovskii Institute for Parasitology and Tropical MedicineSechenov Moscow Medical AcademyMoscowRussia

Personalised recommendations