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Increase in glucoamylase productivity of Aspergillus awamori strain by combination of radiation mutagenesis and plasmid transformation methods

Abstract

Increase in the expression level of amylolytic genes activator protein encoded by amyR gene was shown to result in enhancement of glucoamylase productivity of A. awamori strain by 30%. However, the same effect equal to 30% increase can be achieved by introduction of extra copies of gla gene encoding glucoamylase. These two effects were not additive, which gave the possibility to suggest an additional limitation in the regulation mechanism of glucoamylase gene expression in A. awamori strain while introducing an additional copies of amyR and gla genes.

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References

  1. 1.

    US Patent no. 5358864, 1994.

  2. 2.

    US Patent no. 5298405, 1994.

  3. 3.

    US Patent no. 4 966 850, 1990.

  4. 4.

    US Patent no. 5610048, 1997.

  5. 5.

    EU Patent no. 0420358A1, 1990.

  6. 6.

    WIRO Patent no. WO9855599A2, 1998.

  7. 7.

    Nikolaev, I.V., Bekker, O.B., Serebryanyi, V.A., Chulkin, A.M., and Vinetskii, Yu.P., Biotekhnologiya, 1999, no. 3, pp. 3–13.

  8. 8.

    Serebryanyi, V.A., Vavilova, E.A., Chulkin, A.M., and Vinetskii, Yu.P., Prikl. Biokhim. Mikrobiol., 2002, vol. 38, no. 5, pp. 495–501 [Appl. Biol. (Eng. Transl.), 2002, vol. 38, no. 5, pp. 420–426].

  9. 9.

    Serebryanyi, V.A., Sinitsyna, O.A., Fedorova, E.A., Okunev, O.N., Bekkarevich, A.O., Sokolova, L.M., Vavilova, E.A., Vinetsky, Yu.P., and Sinitsyn, A.P., Prikl. Biokhim. Mikrobiol., 2006, vol. 42, no. 6, pp. 665–673 [Appl. Biol. (Eng. Transl.), 2006, vo. 42, no. 6, pp. 584–591].

  10. 10.

    Vinetsky, Yu.P., Rozhkova, A.M., Chulkin, A.M., Satrutdinov, A.D., Sinitsyna, O.A., Fedorova, E.A., Bekkarevich, A.O., Okunev, O.N., and Sinitsyn, A.P., Biokhimiya, 2009, vol. 74, no. 8, pp. 1084–1090 [Biochemistry (Moscow), 2009, vol. 74, no. 8, pp. 882–887].

  11. 11.

    Gomi, K., Akeno, T., Minetoki, T., Ozeki, K., Kumagai, C., and Iimura, Y., Biotechnol. Biochem., 2000, vol. 64, pp. 816–827.

  12. 12.

    Pel, H.J., de Winde, J.H., Archer, D.B., Dyer, P.S., Hofmann, G., Schaap, P.J., Turner, G., et al., Nat. Biotechnol., 2007, vol. 25, no. 2, pp. 221–231.

  13. 13.

    Aleksenko, A.Y., Makarova, N.A., Nikolaev, I.V., and Clutterbuck, A.J., Curr. Genet., 1995, vol. 28, pp. 474–478.

  14. 14.

    Punt, P.J., Oliver, R.P., Dingemanse, M.A., Pouwels, P.H., and Hondel, C.A., Gene, 1987, vol. 56, pp. 117–124.

  15. 15.

    Aslanidis, C. and de Jong, J.P., Nucleic Acids Res., 1990, vol. 18, pp. 6069–6075.

  16. 16.

    Tilburn, J., Scazzocchio, C., Taylor, G.G., Zabicky-Zissman, J.H., Lockington, R.A., and Davies, R.W., Gene, 1983, vol. 26, pp. 205–221.

  17. 17.

    Semenova, M.V., Zorov, I.N., Sinitsyn, A.P., Okunev, O.N., Baryshnikova, L.M., and Tsurikova, N.V., Mikrobnye biokatalizatory dlya pererabatyvayushchikh otraslei APK (Microbial Biocatalysts for Processing Branches of Industry of APC), Polyakov, V.A. and Rimarev, L.V., Eds., Moscow: Pishchepromizdat, 2006.

  18. 18.

    Nunberg, J.H., Meade, J.H., Cole, G., Lawyer, F.C., McCabe, P., Shweickart, V., Tal, R., Wittman, V.P., Flatgaard, J.E., and Innis, M.A., Mol. Cell. Biol., 1984, vol. 4, no. 11, pp. 2306–2315.

  19. 19.

    Boel, E., Hjort, I., Svensson, B., Norris, F., and Norris, K., EMBO J., 1984, vol. 3, no. 5, pp. 1097–1102.

  20. 20.

    Verdoes, J.C., Punt, P.J., Stouthamer, A.H., and Hondel, C.A.M.J.J., Gene, 1994, vol. 145, pp. 179–187.

  21. 21.

    Tsukagoshi, N., Kobayashi, T., and Kato, M., J. Gen. Appl. Microbiol., 2001, vol. 47, pp. 1–19.

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Original Russian Text © Yu.P. Vinetsky, A.M. Rozhkova, A.S. Sereda, N.V. Tsurikova, A.K. Nurtaeva, M.V. Semenova, I.N. Zorov, A.P. Sinitsyn, 2010, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2010, Vol. 46, No. 6, pp. 685–692.

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Vinetsky, Y.P., Rozhkova, A.M., Sereda, A.S. et al. Increase in glucoamylase productivity of Aspergillus awamori strain by combination of radiation mutagenesis and plasmid transformation methods. Appl Biochem Microbiol 46, 633–640 (2010). https://doi.org/10.1134/S0003683810060128

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Keywords

  • Apply Biochemistry
  • Isomaltose
  • Plasmid Transformation
  • Glucoamylase Productivity
  • Proto Plasts