Applied Biochemistry and Microbiology

, Volume 46, Issue 6, pp 633–640 | Cite as

Increase in glucoamylase productivity of Aspergillus awamori strain by combination of radiation mutagenesis and plasmid transformation methods

  • Yu. P. Vinetsky
  • A. M. Rozhkova
  • A. S. Sereda
  • N. V. Tsurikova
  • A. K. Nurtaeva
  • M. V. Semenova
  • I. N. Zorov
  • A. P. Sinitsyn


Increase in the expression level of amylolytic genes activator protein encoded by amyR gene was shown to result in enhancement of glucoamylase productivity of A. awamori strain by 30%. However, the same effect equal to 30% increase can be achieved by introduction of extra copies of gla gene encoding glucoamylase. These two effects were not additive, which gave the possibility to suggest an additional limitation in the regulation mechanism of glucoamylase gene expression in A. awamori strain while introducing an additional copies of amyR and gla genes.


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© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  • Yu. P. Vinetsky
    • 1
  • A. M. Rozhkova
    • 1
  • A. S. Sereda
    • 2
  • N. V. Tsurikova
    • 2
  • A. K. Nurtaeva
    • 3
  • M. V. Semenova
    • 1
  • I. N. Zorov
    • 1
  • A. P. Sinitsyn
    • 4
  1. 1.Bakh Institute of BiochemistryRussian Academy of SciencesMoscowRussia
  2. 2.Institute of Food BiotechnologyRussian Academy of Agricultural SciencesMoscowRussia
  3. 3.Institute of Management and Economy of KazakhstanAlma AtyKazakhstan
  4. 4.Department of ChemistryMoscow State UniversityMoscowRussia

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