Advertisement

Applied Biochemistry and Microbiology

, Volume 45, Issue 4, pp 454–458 | Cite as

Gibberellin A3 detection in plants with high-performance liquid chromatography

  • E. E. Nefed’evaEmail author
  • N. G. Mazey
Article

Abstract

A new method of gibberellin A3 detection based on high-performance liquid chromatography has been elaborated. It combines high accuracy and low cost, and includes the following stages: fixation with liquid nitrogen; extraction of alcohol-soluble substances; purification with freezing, reextraction in ethyl acetate, and thin-layer chromatography; and detection with HPLC. Chromatographic conditions and methods of substance identification have been determined. The method allows the use of modest amounts of plant material (5–20 g), at which variability does not exceed 7%, and the total loss of substance is approximately 3%. The results of gibberellin A3 detection in buckwheat are presented.

Keywords

Apply Biochemistry Gibberellin Substance Loss Typical Chromatogram Lactuca Sativa 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Dörffling, K., Das Hormonsystem der Pflanzen, Stuttgart: Thieme, 1982.Google Scholar
  2. 2.
    Berezkin, V.G., Khimicheskie metody v gazovoi khromatografii (Chemical Methods in Gas Chromatography), Moscow: Khimiya, 1980.Google Scholar
  3. 3.
    Wolbang, C.M., Chandler, P.M., Smith, J.J., and Ross, J.J., Plant Physiol., 2004, vol. 134, no. 2, pp. 769–776.PubMedCrossRefGoogle Scholar
  4. 4.
    Lozhnikova, V.N., Khlopenkova, L.P., and Chailakhyan, M.Kh., Metody opredeleniya fitogormonov, ingibitorov rosta, defoliantov i gerbitsidov (Methods of Determination of Phytohormones, Growth Inhibitors, Defoliants, and Herbicides), Rakitin, Yu.V., Ed., Moscow: Nauka, 1973, pp. 50–58.Google Scholar
  5. 5.
    Dauphin, B., Teller, G., and Durand, B., Planta, 1979, vol. 144, no. 2, pp. 113–119.CrossRefGoogle Scholar
  6. 6.
    Myasoedov, N.F., Sidorov, G.V., and Lushkina, O.V., RF Patent no. 2076318C1, 6G01 N30/06, 1997.Google Scholar
  7. 7.
    Sakodynskii, K.I., Brazhnikov, V.V., Volkov, S.A., Zel’venskii, V.Yu., Gankina, E.S., and Shatts, V.D., Analiticheskaya khromatografiya (Analytical Chromatography), Moscow: Khimiya, 1993.Google Scholar
  8. 8.
    Shmidt, V.M., Matematicheskie metody v botanike (Mathematical Methods in Botany), Leningrad: Leningr. Gos. Univ., 1984.Google Scholar
  9. 9.
    Muromtsev, G.S., Chkannikov, D.I., Kulaeva, O.N., and Gamburg, K.Z., Osnovy khimicheskoi regulyatsii rosta i productivnosti rastenii (Chemical Regulation of Plant Growth and Productivity), Moscow: Agropromizdat, 1987.Google Scholar
  10. 10.
    Atzorn, R. and Weiler, E.W., Planta, 1983, vol. 159, no. 1, p. 7.CrossRefGoogle Scholar
  11. 11.
    Kholodar’, V.A., Shevtsov, S.V., and Chekurov, V.M., Fiziol. Rast., 1995, vol. 42, pp. 647–651.Google Scholar
  12. 12.
    Kaneko, M., Itoh, H., Ueguchi-Tanaka, M., Ashikari, M., and Matsuoka, M., Plant Physiol., 2002, vol. 128, no. 4, pp. 1264–1270.PubMedCrossRefGoogle Scholar
  13. 13.
    Toyomasu, T., Kawaide, H., Mitsuhashi, W., Inoue, Y., and Kamiya, Y., Plant Physiol., 1998, vol. 118, no. 4, pp. 1517–1523.PubMedCrossRefGoogle Scholar
  14. 14.
    Ikeda, A., Sonoda, Y., Vernieri, P., Perata, P., Hirochika, H., and Yamaguchi, J., Plant Cell Physiol., 2002, vol. 43, no. 9, pp. 974–979.PubMedCrossRefGoogle Scholar
  15. 15.
    Iwasaki, Y., Fujisawa, Y., and Kato, H., J. Plant Growth Regul., 2003, vol. 22, no. 2, pp. 126–133.CrossRefGoogle Scholar
  16. 16.
    Debeaujon, I. and Koornneef, M., Plant Physiol., 2000, vol. 122, no. 2, pp. 415–424.PubMedCrossRefGoogle Scholar
  17. 17.
    Wu, C.-T., Leubner-Metzger, G., Meins, F., and Bradford, K.J., Plant Physiol., 2001, vol. 126, no. 6, pp. 1299–1313.PubMedCrossRefGoogle Scholar
  18. 18.
    Filardo, F.F. and Swain, S.M., J. Plant Growth Regul., 2003, vol. 22, no. 2, pp. 163–175.CrossRefGoogle Scholar
  19. 19.
    Styskin, E.L., Itsikson, L.B., and Braude, E.V., Prakticheskaya vysokoeffektivnaya zhidkostnaya khromatografiya (Practical High-Performance Liquid Chromatography), Moscow: Khimiya, 1986.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  1. 1.Volgograd State Technical UniversityVolgogradRussia
  2. 2.Penza State Pedagogical UniversityPenzaRussia

Personalised recommendations