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Growth of Methylosinus trichosporium OB3b on methane and poly-β-hydroxybutyrate biosynthesis

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Abstract

Optimal conditions for batch cultivation of the obligate methanotroph Methylosinus trichosporium OB3b on methane without superatmospheric pressure were chosen. The yield of absolutely dry biomass after 120 h of growth reached 20 g/l. This biomass contained 30% poly-β-hydroxybutyrate (PHB) with molecular weight 300 kDa. The growth process included the stages of biomass growth and PHB biosynthesis. The latter stage occurred under nitrogen-deficiency conditions. It was accompanied by an increase in the activity of PHB biosynthesis enzymes (β-ketothiolase, acetoacetyl-CoA reductase, and PHB synthase) and the main NAD(P)H producer, methylenetetrahydromethanopterin dehydrogenase. The activity of PHB depolymerase increased insignificantly.

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Correspondence to Yu. A. Trotsenko.

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Original Russian Text © N.V. Doronina, V.A. Ezhov, Yu.A. Trotsenko, 2008, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2008, Vol. 44, No. 2, pp. 202–206.

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Doronina, N.V., Ezhov, V.A. & Trotsenko, Y.A. Growth of Methylosinus trichosporium OB3b on methane and poly-β-hydroxybutyrate biosynthesis. Appl Biochem Microbiol 44, 182–185 (2008). https://doi.org/10.1134/S0003683808020099

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Keywords

  • Apply Biochemistry
  • Methanotrophs
  • Methylocystis
  • Methylosinus
  • Serine Pathway