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A novel approach to studying the structural and functional properties of proteins with unknown functions

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Abstract

Three proteins from extremophilic bacteria—hypothetical monooxygenase from Deinococcus radiodurans, hypothetical nucleotidyl transferase from Thermotoga maritime, and hypothetical oxidoreductase from Exiguobacterium sibiricum—and the DJ-1 chaperone protein from Homo sapiens have been produced in Escherichia coli. The isolation and purification procedures developed for the recombinant proteins allowed us to achieve yields higher than 96%. Crystallization conditions enabling stable growth of crystals have been determined. X-ray experiments have been performed to test the quality of the crystals and the resolution achieved ranged from 1.2 to 1.8 Å.

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Abbreviations

MPD:

2-methyl-2,4-pentanediol

PEG:

polyethylene glycol

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Correspondence to M. A. Gorbacheva.

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Original Russian Text © M.A. Gorbacheva, A.G. Yarosh, P.V. Dorovatovskii, T.V. Rakitina, K.M. Boiko, D.A. Korzhenevskii, A.V. Lipkin, V.O. Popov, I.A. Shumilin, 2012, published in Bioorganicheskaya Khimiya, 2012, Vol. 38, No. 1, pp. 99–105.

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Gorbacheva, M.A., Yarosh, A.G., Dorovatovskii, P.V. et al. A novel approach to studying the structural and functional properties of proteins with unknown functions. Russ J Bioorg Chem 38, 83–88 (2012). https://doi.org/10.1134/S1068162012010098

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  • DOI: https://doi.org/10.1134/S1068162012010098

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