Biointerphases

, Volume 6, Issue 4, pp 153–157 | Cite as

Proteopolymersomes: In vitro production of a membrane protein in polymersome membranes

  • Madhavan Nallani
  • Mirjam Andreasson-Ochsner
  • Cherng-Wen Darren Tan
  • Eva-Kathrin Sinner
  • Yudi Wisantoso
  • Susana Geifman-Shochat
  • Walter Hunziker
Open Access
Article

Abstract

Polymersomes are stable self-assembled architectures which mimic cell membranes. For characterization, membrane proteins can be incorporated into such bio-mimetic membranes by reconstitution methods, leading to so-called proteopolymersomes. In this work, we demonstrate the direct incorporation of a membrane protein into polymersome membranes by a cell-free expression system. Firstly, we demonstrate pore formation in the preformed polymersome membrane using α-hemolysin. Secondly, we use claudin-2, a protein involved in cell-cell interactions, to demonstrate the in vitro expression of a membrane protein into these polymersomes. Surface plasmon resonance (Biacore) binding studies with the claudin-2 proteopolymersomes and claudin-2 specific antibodies are performed to show the presence of the in vitro expressed protein in polymersome membranes.

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Copyright information

© American Vacuum Society 2011

Authors and Affiliations

  • Madhavan Nallani
    • 1
  • Mirjam Andreasson-Ochsner
    • 1
  • Cherng-Wen Darren Tan
    • 1
  • Eva-Kathrin Sinner
    • 1
  • Yudi Wisantoso
    • 2
  • Susana Geifman-Shochat
    • 2
  • Walter Hunziker
    • 3
  1. 1.Institute of Materials Research and EngineeringA*STAR (Agency for Science, Technology and Research)Singapore
  2. 2.School of Biological SciencesNanyang Technological UniversitySingapore
  3. 3.Institute of Molecular and Cell BiologyA*STAR (Agency for Science, Technology and Research)Singapore

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