Abstract
The conformational changes in elastinlike polypeptides (ELPs) grafted to a solid/solution interface via different architectures were studied using surface plasmon resonance spectroscopy and surface plasmon field-enhanced fluorescence spectroscopy (SPFS). SPFS provides a simple and convenient optical method to study the influence of the grafting method and the graft density on the conformational changes in ELPs at the solid-solution interface as a function of environmental variables. A typical response of the ELP, consistent with its stimuli responsiveness, was a gradual collapse upon increasing the ionic strength; this effect was inversely correlated with the surface graft density of the ELP.
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Xu, F., Joon, H.M., Trabbic-Carlson, K. et al. Surface plasmon optical study of the interfacial phase transition of elastinlike polypeptide grafted on gold. Biointerphases 3, 66–74 (2008). https://doi.org/10.1116/1.2965133
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DOI: https://doi.org/10.1116/1.2965133