Abstract
We succeeded in primary culture of 3 in 4 cases of glioblastomas. The long-term passage cultures were not done from the primary cultures of original tumor, but glioblastoma cell line (HUBT-n) was established from a xenograft of nude mouse. This line grew well without interruption for 4 years and was subcultivated over 120 times. The cells were spindle like or round in shape and neoplastic and pleomorphic features contained glial fibrillar acid protein (GFAP) and S-100 protein and grew multilayering without contact inhibition. A bough-shaped long projection was noted from a small cell. One of the characteristics of the HUBT-n cells was existence of well developed intermediate filaments in their cytoplasm. The cells proliferated rapidly, and the population doubling time was about 32 hours. The chromosome number showed a narrow distribution of diploid range. Abnormal constitution was observed in all cells by G-band karyotyping. The culture cells were easily transplanted into the subcutis of nude mouse and produced the tumor resembling the original tumor.
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References
Nuki I, Uchinokura S, Miyata S. et al.: Establishment and characterization of a new human glioblastoma cell line, HYGM. Hum Cell 17: 135–140, 2004.
Moriyama T, Kataoka H, Seguchi K, et al.: Establishment of a new human glioblastoma cell line (HGM-1) with highly motil phenotype. Hum Cell 10: 105–110, 1997.
Itoh H, Waga S, Kusano I, et al.: GFAP positive cell line established from recurrent malignant glioma. Hum Cell 3: 249–250, 1990.
Onda K, Washiyama K, Tanaka R, et al.: GFAP-positive human glioma cell lines: no.11. no.10. Hum Cell 3: 251–252, 1990.
Kuroiwa T, Kobata H, Fujii A, et al.: Establishment and characterization of malignant glioma cell line. Hum Cell 3: 253–254, 1990.
Takeshita I, Yakai T, Nakamura T, et al.: Established cell lines derived from human gliomas. Hum Cell 3: 255–256, 1990.
Lee S, Iryo O, Sugita Y, et al.: Characterization of an established human malignant glioma cell line:KALA-1. Hum Cell 3: 259–260, 1990.
Morimura T, Matsumoto T, Kaba K, et al.: Establishment of human glioma cell line: GB-1. Hum Cell 3: 261–262, 1990.
Todo T, Kondo T, Yamada K, et al.: Establishment and characterization of a human glioma cell line: NMCG-1. Hum Cell 7: 72–73, 1994.
Izumi I, Mineura K, Watanabe K, et al.: Human glioma cell lines: YH and AM. Hum Cell 7: 74–75, 1994.
Tsuboi K, Yoshii Y and Nose T: Establishment of human glioblastoma cell line “TK-1”. Hum Cell 9: 125–128, 1996.
Kanda N, Tsuchida Y, Hata J, et al.: Amplofication of IMR-32 clones 8, G21, and N-myc in human neuroblastoma xenografts. Cancer Res 47: 3291–3295, 1987.
Kohl NE, Kanda N, Schreck RR, et al.: Transposition and amplification of oncogene-related sequences in human neuroblastomas. Cell 35: 359–367, 1983.
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Ishiwata, I., Ishiwata, C., Ishiwata, E. et al. Establishment and characterization of human glioblastoma cell line (HUBT-n). Hum Cell 18, 59–65 (2005). https://doi.org/10.1111/j.1749-0774.2005.tb00058.x
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DOI: https://doi.org/10.1111/j.1749-0774.2005.tb00058.x