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Establishment and characterization of human malignant lymphoma cell line derived from uterine cervix

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Abstract

Human uterine cervical malignant lymphoma (B-cell type) was cultured and the cell line (HIUML) was newly established. The HIUML cells were round in shape and had a tendency to make floating clusters. The cells had a smooth surface or protrusion on the margin of the cytoplasm, and proliferate in floatation. The population doubling time was about 32 hours and 42 or more passages were successfully observed in two years. The HIUML cells were not transplantable into nude mice but were successfully done in the cheek pouch of hamster with formation of malignant lymphoma. Epstein-Barr virus was detected in the HIUML cells.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, St.Marianna University School of Medicine, Japan

    Kazushige Kiguchi, Masanori Iwata, Yoshiko Okuda, Youichi Kobayashi, Yasuna Wada & Bunpei Ishizuka

  2. Ishiwata Obstet. & gynec. Hospital, 1-4-21, Kami-Mito, Mito-shi, 310-0041, Ibaraki-ken, Japan

    Isamu Ishiwata & Chieko Ishiwata

  3. Department of Obstetrics and Gynecology, Fujita Health University School of Medicine, Japan

    Emiko Ishiwata

  4. Department of Pathology, Machida Municipal Hospital, Japan

    Yutaka Koshitaka

  5. Department of Anatomy, School of medicine, Jikei University, Japan

    Hiroshi Ishikawa

Authors
  1. Kazushige Kiguchi
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  2. Isamu Ishiwata
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  3. Chieko Ishiwata
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  4. Emiko Ishiwata
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  5. Yutaka Koshitaka
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  6. Masanori Iwata
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  7. Yoshiko Okuda
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  8. Youichi Kobayashi
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  9. Yasuna Wada
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  10. Bunpei Ishizuka
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  11. Hiroshi Ishikawa
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Kiguchi, K., Ishiwata, I., Ishiwata, C. et al. Establishment and characterization of human malignant lymphoma cell line derived from uterine cervix. Hum Cell 18, 53–58 (2005). https://doi.org/10.1111/j.1749-0774.2005.tb00057.x

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  • DOI: https://doi.org/10.1111/j.1749-0774.2005.tb00057.x

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