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Establishment and characterization of a human liposarcoma cell line (HTLS) from the retroperitoneal liposarcoma

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Abstract

A cell line designated HTLS was established from the retroperitoneal liposarcoma. The HTLS line showed stable proliferation without interruption for 2 years and subcultivated over 35 times. The cells were elongated fibrous and spindle in shape, and neoplastic and pleomorphic features. The multinucleated giant cells with he cytoplasm were seen. The cells proliferated slowly and the population doubling time was about 90 hours. The chromosome number showed a wide distribution of aneuploidy, the mode was hyperdiploid range (51–52), and many marker chromosomes were observed. The cells were transplantable into the submucosa of immunesuppressed hamster’s cheek pouch and produced liposarcoma, while were not transplantable into subcutis of nude mice

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Authors and Affiliations

  1. Department of Obstetrics & Gynecology, St. Marianna University, Japan

    Kazushige Kiguchi, Tatsuru Ohara, Yoshiko Okuda, Sojiro Sato, Youichi Kobayashi & Bunpei Ishizuka

  2. Ishiwata Obstetrics & Gynecology Hospital, Japan

    Isamu Ishiwata

  3. Department of Obstetrics and Gynecology, Fujita Health University School of Medicine, Japan

    Emiko Ishiwata

  4. Department of Pathology, Machida Municipal Hospital, Japan

    Yutaka Koshitaka

  5. Department of Anatomy, Jikei University, School of Medicine, Japan

    Hiroshi Ishikawa

Authors
  1. Kazushige Kiguchi
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  2. Isamu Ishiwata
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  3. Emiko Ishiwata
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  4. Yutaka Koshitaka
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  5. Tatsuru Ohara
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  6. Yoshiko Okuda
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  7. Sojiro Sato
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  8. Youichi Kobayashi
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  9. Bunpei Ishizuka
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  10. Hiroshi Ishikawa
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Kiguchi, K., Ishiwata, I., Ishiwata, E. et al. Establishment and characterization of a human liposarcoma cell line (HTLS) from the retroperitoneal liposarcoma. Hum Cell 18, 45–52 (2005). https://doi.org/10.1111/j.1749-0774.2005.tb00056.x

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  • DOI: https://doi.org/10.1111/j.1749-0774.2005.tb00056.x

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