Abstract
An intercostal nerve obtained from a human cadaver 6 h post-mortem was transplanted into the rat sciatic nerve and nerve regeneration was observed 4 and 8 weeks after surgery. Sciatic nerves from deceased rats up to 2 days post-mortem were also transplanted for comparison. Good nerve regeneration was observed through the human cadaver-derived graft to the distal segment at the medial plantal nerve 8 weeks after surgery. The results of the present study indicate the possibility that nerves from human cadavers can be used for nerve grafting in clinical applications.
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References
Fansa H, Schneider W, Wolf G, Keilhoff G (2002) Host responses after acellular muscle basal lamina allografting used as a matrix for tissue engineered nerve grafts1. Transplantation 74, 381–7.
Fox IK, Jaramillo A, Hunter DA, Rickman SR, Mohanakumar T, Mackinnon SE (2005) Prolonged cold-preservation of nerve allografts. Muscle Nerve 31, 59–69.
Fujimoto E, Miki A, Ide C (1992) Regenerating axons in basal lamina tubes of non-neural tissue. Biomed Res 13, 59–68.
Fujimoto E, Mizoguchi A, Hanada K, Yajima M, Ide C (1997) Basic fibroblast growth factor promotes extension of regenerating axons of peripheral nerve. In vivo experiments using a Schwann cell basal lamina tube model. J Neurocytol 26, 511–28.
Hashimoto T, Suzuki Y, Kitada M et al. (2002) Peripheral nerve regeneration through alginate gel: Analysis of early outgrowth and late increase in diameter of regenerating axons. Exp Brain Res 146, 356–68.
Hudson TW, Zawko S, Deister C et al. (2004) Optimized acellular nerve graft is immunologically tolerated and supports regeneration. Tissue Eng 10, 1641–51.
Ide C (1984) Nerve regeneration through the basal lamina scaffold of the skeletal muscle. Neurosci Res 1, 379–91.
Ide C (1996) Peripheral nerve regeneration. Neurosci Res 25, 101–21.
Ide C, Tohyama K, Yokota R, Nitatori T, Onodera S (1983) Schwann cell basal lamina and nerve regeneration. Brain Res 288, 61–75.
Ikeguchi R, Kakinoki R, Okamoto T, Matsumoto T, Hyon SH, Nakamura T (2003) Successful storage of peripheral nerve before transplantation using green tea polyphenol: An experimental study in rats. Exp Neurol 184, 688–96.
Kerns JM, Danielsen N, Zhao Q, Lundborg G, Kanje M (2003) A comparison of peripheral nerve regeneration in acellular muscle and nerve autografts. Scand J Plast Reconstr Surg Hand Surg 37, 193–200.
Kim BS, Yoo JJ, Atala A (2004) Peripheral nerve regeneration using acellular nerve grafts. J Biomed Mater Res A 68, 201–9.
Lundborg G (2004) Alternatives to autologous nerve grafts. Review. Handchir Mikrochir Plast Chir 36, 1–7.
Meek MF, Varejao AS, Geuna S (2004) Use of skeletal muscle tissue in peripheral nerve repair: Review of the literature. Tissue Eng 10, 1027–36.
Mligiliche N, Kitada M, Ide C (2001) Grafting of detergent- denatured skeletal muscles provides effective conduits for extension of regenerating axons in the rat sciatic nerve. Arch Histol Cytol 64, 29–36.
Mligiliche N, Endo K, Okamoto K, Fujimoto E, Ide C (2002) Extracellular matrix of human amnion manufactured into tubes as conduits for peripheral nerve regeneration. J Biomed Mater Res 63, 591–600.
Mligiliche NL, Tabata Y, Kitada M et al. (2003) Poly lactic acid-caprolactone copolymer tube with a denatured skeletal muscle segment inside as a guide for peripheral nerve regeneration: A morphological and electrophysiological evaluation of the regenerated nerves. Anat Sci Int 78, 156–61.
Nakamura T, Inada Y, Fukuda S et al. (2004) Experimental study on the regeneration of peripheral nerve gaps through a polyglycolic acid-collagen (PGA-collagen) tube. Brain Res 1027, 18–29.
Osawa T, Tohyama K, Ide C (1990) Allogeneic nerve grafts in the rat, with special reference to the role of Schwann cell basal laminae in nerve regeneration. J Neurocytol 19, 833–49.
Suzuki K, Suzuki Y, Tanihara M et al. (2000) Reconstruction of rat peripheral nerve gap without sutures using freeze-dried alginate gel. J Biomed Mater Res 49, 528–33.
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Fujimoto, E., Ohshima, C., Okamoto, K. et al. Possibility of using nerve segments dissected from human cadavers for grafting: Preliminary report. Anato Sci Int 81, 34–38 (2006). https://doi.org/10.1111/j.1447-073X.2006.00132.x
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DOI: https://doi.org/10.1111/j.1447-073X.2006.00132.x