Abstract
Experimental insemination was performed using artificially produced low-motility sperm. A mathematical model was applied to the results of the insemination in order to clarify the relationship between sperm motility, the density of sperm and the fertilization rate of eggs. In the model, the probability of fertilization by individual spermatozoa was a function of sperm density in the insemination solution. The results showed that the probability of fertilization clearly decreased with increased sperm density, and the maximum possible fertilizing rate by increasing the sperm density was constrained by the proportion of motile sperm (% motility). The model was also applied to the results of insemination tests of cryopreserved sperm in order to evaluate the fertilizing capacity of cryopreserved sperm. It was proven that cryopreserved sperm needed a higher density to obtain the maximum fertilization rate compared with fresh sperm, and it was anticipated that the ratio of the motile inseminated cryopreserved sperm should be more than 5.0% to achieve an egg fertilization rate greater than 90%.
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References
Stein H, Bayrle H. Cryopreservation of the sperm of some teleosts. Ann. Biol. Anim. Bioch. Biophys. 1978; 18: 1073–1076.
Kurokura H, Hirano R. Cryopreservation of rainbow trout sperm. Nippon Suisan Gakkaishi 1980; 46: 1493–1495.
Leung LKB, Jamieson BGM. Live preservation of fish gametes. In: Jamieson BGM (ed.). Fish Euolution and Systematics. Cambridge University Press. Cambridge. 1991; 245–269.
Rana K. Preservation of gametes. In: Bromage N, Roberts R (eds). Broodstock Management and Egg and Larval Quality. Blackwell Scientific Publications, Oxford. 1995; 53–57.
Tiersch TR, Mazik PM. Cryopreservation in Aquatic Species. World Aquaculture Society, Baton Rouge, LA. 2000.
Lahnsteiner F. Semen cryopreservation in the Salmonidae and in the northern pike. Aquacult. Res. 2000; 31: 245–258.
Stoss J, Holtz W. Cryopreservation of rainbow trout (Salmo gairdneri) sperm. I. Effect of thawing solution, sperm density and interval between thawing and insemination. Aquaculture 1981; 22: 97–104.
Levanduski MJ, Cloud JG. Rainbow trout (Salmo gairdneri) semen: effect of non-motile sperm on fertility. Aquaculture 1988; 75: 171–179.
Stoss J, Refstie T. Short term storage and cryopreservation of milt from Atlantic salmon and sea trout. Aquaculture 1983; 30: 229–236.
Billard R, Cosson MP. Some problems related to the assessment of sperm motility in freshwater fish. J. Exp. Zoolog. 1992; 261: 122–131.
Morisawa M, Suzuki K. Osmolality and potassiumion. Their roles in initiation of sperm motility in teleosts. Science 1980; 210: 1145–1147.
Minagawa M, Sakai K, Takashima F. Motility and other characteristics of the sperm of rainbow trout. Suisanzoushoku 1986; 34: 199–200.
Ohta H, Matsubara T, Imada K. Sperm motility of masu Salmon, Oncorhynchus masou, in coelomic and artificial coelomic fluid. Suisanzoushoku 1989; 37: 235–239.
Scott AP, Baynes SM. A review of the biology, handling and storage of salmonid spermatozoa. J. Fish Biol. 1980; 17: 707–739.
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Kurokura, H., Oo, K.M. Evaluation of fertilizing capacity of cryopreserved rainbow trout sperm. Fish Sci 74, 621–626 (2008). https://doi.org/10.1111/j.1444-2906.2008.01566.x
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DOI: https://doi.org/10.1111/j.1444-2906.2008.01566.x