Availability of genetically modified feed ingredient: investigations of ingested foreign DNA in rainbow trout Oncorhynchus mykiss
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Foreign DNA fragments from genetically modified defatted soybean meal (GM SEM) in rainbow trout was traced by nested polymerase chain reaction (PCR) and located by in situ hybridization. Either a GM or non-GM SBM formulated diet (42% protein) was fed to fish (average weight 50.5 g) for 2 weeks. The degradation results showed that the cauliflower mosaic virus 35S promoter (220 bp) fragment was detected in the contents of digestive system only in fish fed the GM SBM diet, and it was not detected on the third day after changing the diet to the non-GM SBM diet. For the possible transferal results, the promoter fragment was detected in the leukocyte, head kidney and muscle only of fish fed the GM SBM diet; it was not detected on the fifth day after changing the diet to the non-GM SBM diet. These results suggest that a foreign DNA fragment was not completely degraded and might be taken up into organs through the gastrointestinal tract. However, foreign DNA was not detected after the withdrawal period. Thus, the data show that uptake of DNA from GM SBM might not remain in the tissues of fish fed GM SBM diet.
Key WordsCaMV 35S promoter gastrointestinal tract genetically modified defatted soybean meal in situ hybridization
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- 2.Cowx IG. Cultured Aquatic Species Information Programme — Oncorhynchus mykiss. [Cited 22 June 2006] Available from URL: http://www.fao.org/fishery/culturedspecies/Oncorhynchus_mykissGoogle Scholar
- 5.Padgette SR, Kolacz KH, Delannay X, Re DB, LaVellee BJ, Tinius CN, Rhodes WK, Otero YI, Barry GF, Eichholtz DA, Peschke VM, Nida DL, Taylor NB, Kishore GM. Development, identification and characterization of glyphosatetolerant soybean line. Crop Sci. 1995; 35: 1451–1461.Google Scholar
- 12.Reuter T, Aulrich K. Investigations on genetically modified maize (Bt-maize) in pig nutrition: fate of feed-ingested foreign DNA in pig bodies. Eur. Food Res. Technol. 2003; 216: 185–192.Google Scholar
- 20.Ogino C, Takeuchi L, Takeda H, Watanabe T. Availability of dietary phosphorus in carp and rainbow trout. Nippon Suisan Gakkaishi 1979; 45: 1527–1532.Google Scholar
- 22.Sambrook J, Russell DW. Molecular Cloning: a Laboratory Manual, Vol. 1. Cold Spring Harbor Laboratory Press, New York, NY. 2001.Google Scholar
- 25.Matsuoka T, Kawashima Y, Akiyama H, Miura H, Goda Y, Sebata T, Isshiki K, Toyoda M, Hino A. A detection method for recombinant DNA from genetically modified soybeans and processed foods containing them. Shokuhin Eiseigaku Zasshi (J. Food Hyg. Soc. Jpn.) 1999; 40: 149–157 (in Japanese).Google Scholar
- 27.Forbes JM, Blair GE, Chiter A, Perks S. Effect of feed processing condition on DNA fragmentation. Scientific Report No. 376 to the Ministry of Agriculture, Fisheries and Food, United Kingdom, 1998.Google Scholar
- 33.Beever DE, Kemp CF. Safety issues associated with the DNA in animal feed derived from genetically modified crops. A review of scientific and regulatory procedures. Nutr. Abstr. Rev. Series B 2000; 70: 175–182.Google Scholar