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Genetic identification of native populations of fluvial white-spotted charr Salvelinus leucomaenis in the upper Tone River drainage

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Abstract

Stocking of exogenous, hatchery-reared white-spotted charr Salvelinus leucomaenis has been conducted throughout much of their range in Honshu Island, Japan, to increase angling opportunities. Although the native char populations are thought to have declined because of hybridization with introduce fish, their distribution and genetic status have been uncertain. Fine population structures of charr in the upper Tone River drainage were examined using mitochondrial DNA and microsatellite analyses so as to clarify the presence of native populations. One common mtDNA haplotype was detected in all populations in the Ohashi River and Watarase River, and four and one tributary populations were monomorphic for such haplotypes, respectively. However, several haplotypes, considered to have originated from stocked hatchery fish, were observed in the stocked and the remaining populations. Judging from the genetic integrity over a fine geographic scale, the former were considered as indicative of native populations and the latter as admixtures with hatchery fish. Comparisons of genetic diversity, deviations from the Hardy-Weinberg equilibrium, principal component analysis, and relatedness estimations based on microsatellite DNA can also provide evidence for distinguishing native populations from those influenced by hatchery fish.

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Authors and Affiliations

  1. Tochigi Prefectural Fisheries Experimental Station, 324-0404, Ohtawara, Tochigi, Japan

    Hitoshi Kubota & Takahide Doi

  2. National Research Institute of Fisheries Science, Fisheries Research Agency, 321-1661, Nikko, Tochigi, Japan

    Shoichiro Yamamoto

  3. Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Minato, 108-8477, Tokyo, Japan

    Seiichi Watanabe

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  1. Hitoshi Kubota
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  2. Takahide Doi
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  3. Shoichiro Yamamoto
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  4. Seiichi Watanabe
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Correspondence to Hitoshi Kubota.

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Kubota, H., Doi, T., Yamamoto, S. et al. Genetic identification of native populations of fluvial white-spotted charr Salvelinus leucomaenis in the upper Tone River drainage. Fish Sci 73, 270–284 (2007). https://doi.org/10.1111/j.1444-2906.2007.01333.x

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  • DOI: https://doi.org/10.1111/j.1444-2906.2007.01333.x

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