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Survey of genetic variation at three microsatellite loci in captive populations of endangered Japanese minnow Aphyocypris chinensis with implications for reduction of inbreeding

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Abstract

The Japanese population of the cyprinid minnow Aphyocypris chinensis is nearing extinction in the wild. The genetic diversity of three microsatellite loci in five captive populations was investigated, and an effective breeding strategy to reduce inbreeding from pairwise relatedness (R xy ) between each captive line is discussed. The average number of alleles ranged 2.33–4.67 and the average heterozygosity ranged 0.283–0.602. The pairwise relatedness observed in most combinations showed a significant decrease between the populations. It is suggested that exchange of individuals between different breeding lines should effectively stop inbreeding. Studies show that the effective population size (N e ) estimated from the number of parental individuals was 8.54 in one captive population, which is insufficient to maintain genetic diversity. It is recommended that more parental individuals should be used, and to exchange fish in a rotating mating mode between institutions participating in captive breeding of A. chinensis.

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Author notes

  1. Kenichi Ohara

    Present address: Gifu Prefectural Research Institute for Freshwater Fish and Aquatic Environments, 501-6021, Kakamigahara, Gifu, Japan

Authors and Affiliations

  1. Lake Biwa Museum, 525-0001, Kusatsu, Shiga, Japan

    Kenichi Ohara

  2. Faculty of Agriculture, Ehime University, 790-8566, Matsuyama, Ehime, Japan

    Motohiro Takagi

Authors
  1. Kenichi Ohara
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  2. Motohiro Takagi
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Correspondence to Motohiro Takagi.

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Ohara, K., Takagi, M. Survey of genetic variation at three microsatellite loci in captive populations of endangered Japanese minnow Aphyocypris chinensis with implications for reduction of inbreeding. Fish Sci 73, 156–160 (2007). https://doi.org/10.1111/j.1444-2906.2007.01313.x

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  • DOI: https://doi.org/10.1111/j.1444-2906.2007.01313.x

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