Fisheries Science

, Volume 72, Issue 6, pp 1286–1291 | Cite as

Inhibitory effects of seaweeds on histamine release from rat basophile leukemia cells (RBL-2H3)

  • Yoshimasa Sugiura
  • Yoshirou Takeuchi
  • Makoto Kakinuma
  • Hideomi AmanoEmail author


The effects of 80% methanol extracts from frozen samples of 41 macroalgae and one sea grass collected in the Ise-Shima region of Japan were investigated on histamine release from rat basophile leukemia cells (RBL-2H3) sensitized with antidinitrophenyl (DNP) IgE and stimulated with DNP-BSA. Of the 21 brown algae, five green and 15 red algae, and one sea grass tested, only extracts from seven brown algae suppressed histamine release from RBL cells, as determined by highperformance liquid chromatography (HPLC) analysis. When the cytotoxic effects of the seven brown algal extracts were investigated by Trypan blue staining, only Eisenia arborea and Sargassum thunbergil did not show cytotoxic effects. Therefore, we conclude that E. arborea and S. thunbergii may contain compounds that have antiallergic effects without inducing cell death.

Key Words

brown algae cytotoxic effect histamine release RBL-2H3 cells 


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Copyright information

© The Japanese Society of Fisheries Science 2006

Authors and Affiliations

  • Yoshimasa Sugiura
    • 1
    • 2
  • Yoshirou Takeuchi
    • 1
  • Makoto Kakinuma
    • 2
  • Hideomi Amano
    • 2
    Email author
  1. 1.Department of TechnologyKanehatsu Food Co. LtdNagoya, AichiJapan
  2. 2.Laboratory of Marine Biochemistry, Graduate School of BioresourcesMie UniversityTsu, MieJapan

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